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- W2794283105 abstract "We previously showed that low levels of melatonin increase nitric oxide (NO) production by PBMC from IgE- adults. As allergy/asthma symptom severity varies with time of day (AM high; PM low), we investigated NO responses of PBMC isolated in AM and PM and their correlation with exhaled NO (FeNO) levels and serum IgE. Blood was collected from IgE+ (>100 IU/ml) (n=12), and IgE- (<100 IU/ml) (n=8) adults at 9-10 AM and 3-4 PM; FeNO and serum IgE were determined (Niox Vero; fluoroenzymeimmunoassay). PBMC were incubated for 5 days ± bradykinin (100 nmol/ml), IL-15 (1 μg/ml), IL-18 (1 μg/ml), IFNγ (10 ng/ml) and Vitamin D3 (20 pmol/ml) (CYT+D3). Recombinant human melatonin (1 pmol/ml) was added for the last 4 hrs of culture, and NO production measured (Griess reaction). Data analysis used Spearman - Pearson coefficients and t-test. PBMC from IgE+ subjects made significantly more NO than IgE- adults when obtained either AM (10.14 ±5.96 μM vs. 3.65 ±0.99 μM, p<0.01) or PM (11.09 ±6.03 μM vs. 3.19 ±1.1 μM, p<0.01). Inclusion of CYT+D3 increased NO production by PBMC obtained PM (from 4.71±4.16 μM to 8.62±6.23 μM (p=0.05) but not AM (p=0.23). NO production correlated with IgE levels in AM and PM (p=0.04, 0.02, respectively), but correlated with FeNO only in PM (p=0.09, 0.02, respectively). NO production by PBMC corresponds to circadian rhythm shown by daily changes in melatonin secretion from pineal gland in the brain. NO production by PBMC may be a useful biomarker in IgE+ adults." @default.
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- W2794283105 date "2018-02-01" @default.
- W2794283105 modified "2023-09-27" @default.
- W2794283105 title "Nitric Oxide Production by PBMC from IgE+ Allergic Adults Varies with Time of Day and Correlates with Total Serum IgE and Exhaled Breath NO" @default.
- W2794283105 doi "https://doi.org/10.1016/j.jaci.2017.12.717" @default.
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