Matches in SemOpenAlex for { <https://semopenalex.org/work/W2794362225> ?p ?o ?g. }
- W2794362225 endingPage "97" @default.
- W2794362225 startingPage "86" @default.
- W2794362225 abstract "Glutaredoxin (Grx) is a polypeptide with low molecular weight, which has been extracted from buckwheat and has been suggested to have multiple functions revolving around oxidative stress responses and cell signaling. Here, we report the antioxidant activity of recombinant buckwheat Grx (rbGrx) to reduce aging effects in Caenorhabditis elegans (C. elegans) as well as the mechanism involved. Our results showed that rbGrx beneficially affected the health span of C. elegans, including pharyngeal-pumping rate, locomotion, and lipofuscin accumulation. Furthermore, stress assay showed that rbGrx could extend the lifespan under both oxidative and heat stress. Further studies indicated that the longevity-extending effects of rbGrx could be attributed to its in vitro and in vivo antioxidant activities. After treatment with rbGrx, SOD activity, CAT activity, GSH content, and GSH/GSSG ratio were increased, while MDA content was decreased, which led to low intracellular levels of reactive oxygen species in C. elegans. Moreover, rbGrx up-regulated hsf-1 mRNA level and could not expand the lifespan of the hsf-1 mutant C. elegans (sy441); however, this had no effect on the transcription of daf-16 and skn-1 and could expand the lifespan of both daf-16 and skn-1 mutants. These results suggested dependency of the rbGrx effect on the heat shock transcription factor (HSF-1) and independency on both DAF-16 and SKN-1. In summary, our results demonstrated the anti-aging activity of rbGrx, which increased resistance to cellular stress and improved the health span of C. elegans. These results are very important for the use of rbGrx in anti-aging research." @default.
- W2794362225 created "2018-03-29" @default.
- W2794362225 creator A5047916176 @default.
- W2794362225 creator A5050271178 @default.
- W2794362225 creator A5063551023 @default.
- W2794362225 creator A5063922467 @default.
- W2794362225 creator A5089852582 @default.
- W2794362225 date "2018-04-01" @default.
- W2794362225 modified "2023-10-08" @default.
- W2794362225 title "Recombinant buckwheat glutaredoxin intake increases lifespan and stress resistance via hsf-1 upregulation in Caenorhabditis elegans" @default.
- W2794362225 cites W1538904872 @default.
- W2794362225 cites W1555938743 @default.
- W2794362225 cites W1558565504 @default.
- W2794362225 cites W1619158228 @default.
- W2794362225 cites W1938310987 @default.
- W2794362225 cites W1940436875 @default.
- W2794362225 cites W1944127002 @default.
- W2794362225 cites W1969399683 @default.
- W2794362225 cites W1970035510 @default.
- W2794362225 cites W1973702181 @default.
- W2794362225 cites W1973973036 @default.
- W2794362225 cites W1974436378 @default.
- W2794362225 cites W1982453959 @default.
- W2794362225 cites W1982647833 @default.
- W2794362225 cites W1986589519 @default.
- W2794362225 cites W1987769373 @default.
- W2794362225 cites W1992390260 @default.
- W2794362225 cites W1993823186 @default.
- W2794362225 cites W1996181447 @default.
- W2794362225 cites W1997242330 @default.
- W2794362225 cites W2000291892 @default.
- W2794362225 cites W2001908075 @default.
- W2794362225 cites W2002794726 @default.
- W2794362225 cites W2002930228 @default.
- W2794362225 cites W2003376000 @default.
- W2794362225 cites W2004112725 @default.
- W2794362225 cites W2008337375 @default.
- W2794362225 cites W2010906156 @default.
- W2794362225 cites W2011277283 @default.
- W2794362225 cites W2015673372 @default.
- W2794362225 cites W2018893523 @default.
- W2794362225 cites W2019227799 @default.
- W2794362225 cites W2023431124 @default.
- W2794362225 cites W2029342214 @default.
- W2794362225 cites W2031358736 @default.
- W2794362225 cites W2032462953 @default.
- W2794362225 cites W2038120788 @default.
- W2794362225 cites W2038977009 @default.
- W2794362225 cites W2039213813 @default.
- W2794362225 cites W2039536853 @default.
- W2794362225 cites W2040359453 @default.
- W2794362225 cites W2041140784 @default.
- W2794362225 cites W2044554559 @default.
- W2794362225 cites W2044726248 @default.
- W2794362225 cites W2046989794 @default.
- W2794362225 cites W2051386839 @default.
- W2794362225 cites W2052275162 @default.
- W2794362225 cites W2052722944 @default.
- W2794362225 cites W2057049100 @default.
- W2794362225 cites W2058127437 @default.
- W2794362225 cites W2058484936 @default.
- W2794362225 cites W2062629127 @default.
- W2794362225 cites W2064412448 @default.
- W2794362225 cites W2065785051 @default.
- W2794362225 cites W2068337326 @default.
- W2794362225 cites W2072117025 @default.
- W2794362225 cites W2073005972 @default.
- W2794362225 cites W2076053153 @default.
- W2794362225 cites W2088731668 @default.
- W2794362225 cites W2093935763 @default.
- W2794362225 cites W2094231976 @default.
- W2794362225 cites W2097783495 @default.
- W2794362225 cites W2098567793 @default.
- W2794362225 cites W2103268140 @default.
- W2794362225 cites W2108791332 @default.
- W2794362225 cites W2109502717 @default.
- W2794362225 cites W2111075771 @default.
- W2794362225 cites W2111223240 @default.
- W2794362225 cites W2111552503 @default.
- W2794362225 cites W2113714326 @default.
- W2794362225 cites W2126525177 @default.
- W2794362225 cites W2126925922 @default.
- W2794362225 cites W2132673369 @default.
- W2794362225 cites W2132826785 @default.
- W2794362225 cites W2133984969 @default.
- W2794362225 cites W2136002979 @default.
- W2794362225 cites W2140591044 @default.
- W2794362225 cites W2141444248 @default.
- W2794362225 cites W2142842603 @default.
- W2794362225 cites W2147993424 @default.
- W2794362225 cites W2148242921 @default.
- W2794362225 cites W2151034892 @default.
- W2794362225 cites W2165178917 @default.
- W2794362225 cites W2166146113 @default.
- W2794362225 cites W2168002177 @default.
- W2794362225 cites W2208569696 @default.
- W2794362225 cites W2315512556 @default.
- W2794362225 cites W2320411846 @default.