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- W2794437835 abstract "As cells with aberrant force-generating phenotypes can directly lead to disease, cellular force-generation mechanisms are high-value targets for new therapies. Here, we show that single-cell force sensors embedded in elastomers enable single-cell force measurements with ~100-fold improvement in throughput than was previously possible. The microtechnology is scalable and seamlessly integrates with the multi-well plate format, enabling highly parallelized time-course studies. In this regard, we show that airway smooth muscle cells isolated from fatally asthmatic patients have innately greater and faster force-generation capacity in response to stimulation than healthy control cells. By simultaneously tracing agonist-induced calcium flux and contractility in the same cell, we show that the calcium level is ultimately a poor quantitative predictor of cellular force generation. Finally, by quantifying phagocytic forces in thousands of individual human macrophages, we show that force initiation is a digital response (rather than a proportional one) to the proper immunogen. By combining mechanobiology at the single-cell level with high-throughput capabilities, this microtechnology can support drug-discovery efforts for clinical conditions associated with aberrant cellular force generation. A microtechnology involving force sensors embedded in elastomers for cell culture enables the high-throughput measurement of single-cell force generation from contractile cells in a scalable and highly parallelized manner." @default.
- W2794437835 created "2018-03-29" @default.
- W2794437835 creator A5025872469 @default.
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- W2794437835 date "2018-02-06" @default.
- W2794437835 modified "2023-10-16" @default.
- W2794437835 title "Elastomeric sensor surfaces for high-throughput single-cell force cytometry" @default.
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- W2794437835 cites W1964063180 @default.
- W2794437835 cites W1972678218 @default.
- W2794437835 cites W1975055486 @default.
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- W2794437835 doi "https://doi.org/10.1038/s41551-018-0193-2" @default.
- W2794437835 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6619436" @default.
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- W2794437835 hasPublicationYear "2018" @default.
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