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• W2794440014 abstract "Saccharomyces cerevisiae wild strains generally have poor xylose-utilization capability, which is a major barrier for efficient bioconversion of lignocellulosic biomass. Laboratory adaption is commonly used to enhance xylose utilization of recombinant S. cerevisiae. Apparently, yeast cells could remodel the metabolic network for xylose metabolism. However, it still remains unclear why natural isolates of S. cerevisiae poorly utilize xylose. Here, we analyzed a unique S. cerevisiae natural isolate YB-2625 which has superior xylose metabolism capability in the presence of mixed-sugar. Comparative transcriptomic analysis was performed using S. cerevisiae YB-2625 grown in a mixture of glucose and xylose, and the model yeast strain S288C served as a control. Global gene transcription was compared at both the early mixed-sugar utilization stage and the latter xylose-utilization stage.Genes involved in endogenous xylose-assimilation (XYL2 and XKS1), gluconeogenesis, and TCA cycle showed higher transcription levels in S. cerevisiae YB-2625 at the xylose-utilization stage, when compared to the reference strain. On the other hand, transcription factor encoding genes involved in regulation of glucose repression (MIG1, MIG2, and MIG3) as well as HXK2 displayed decreased transcriptional levels in YB-2625, suggesting the alleviation of glucose repression of S. cerevisiae YB-2625. Notably, genes encoding antioxidant enzymes (CTT1, CTA1, SOD2, and PRX1) showed higher transcription levels in S. cerevisiae YB-2625 in the xylose-utilization stage than that of the reference strain. Consistently, catalase activity of YB-2625 was 1.9-fold higher than that of S. cerevisiae S288C during the xylose-utilization stage. As a result, intracellular reactive oxygen species levels of S. cerevisiae YB-2625 were 43.3 and 58.6% lower than that of S288C at both sugar utilization stages. Overexpression of CTT1 and PRX1 in the recombinant strain S. cerevisiae YRH396 deriving from S. cerevisiae YB-2625 increased cell growth when xylose was used as the sole carbon source, leading to 13.5 and 18.1%, respectively, more xylose consumption.Enhanced oxidative stress tolerance and relief of glucose repression are proposed to be two major mechanisms for superior xylose utilization by S. cerevisiae YB-2625. The present study provides insights into the innate regulatory mechanisms underlying xylose utilization in wild-type S. cerevisiae, which benefits the rapid development of robust yeast strains for lignocellulosic biorefineries." @default.
• W2794440014 created "2018-03-29" @default.
• W2794440014 creator A5015832863 @default.
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• W2794440014 creator A5052570866 @default.
• W2794440014 creator A5088205358 @default.
• W2794440014 date "2018-02-05" @default.
• W2794440014 modified "2023-10-11" @default.
• W2794440014 title "Association of improved oxidative stress tolerance and alleviation of glucose repression with superior xylose-utilization capability by a natural isolate of Saccharomyces cerevisiae" @default.
• W2794440014 cites W1542925684 @default.
• W2794440014 cites W1896895429 @default.
• W2794440014 cites W1939070248 @default.
• W2794440014 cites W1942529040 @default.
• W2794440014 cites W1971600688 @default.
• W2794440014 cites W1975082668 @default.
• W2794440014 cites W1975589683 @default.
• W2794440014 cites W1976394389 @default.
• W2794440014 cites W1977164941 @default.
• W2794440014 cites W1977523943 @default.
• W2794440014 cites W1977668929 @default.
• W2794440014 cites W1985960090 @default.
• W2794440014 cites W1987551969 @default.
• W2794440014 cites W2007468388 @default.
• W2794440014 cites W2008873787 @default.
• W2794440014 cites W2009188703 @default.
• W2794440014 cites W2009354884 @default.
• W2794440014 cites W2013184614 @default.
• W2794440014 cites W2017361626 @default.
• W2794440014 cites W2025838227 @default.
• W2794440014 cites W2026740559 @default.
• W2794440014 cites W2027296541 @default.
• W2794440014 cites W2031315147 @default.
• W2794440014 cites W2032793812 @default.
• W2794440014 cites W2033639139 @default.
• W2794440014 cites W2038591876 @default.
• W2794440014 cites W2039522748 @default.
• W2794440014 cites W2053300791 @default.
• W2794440014 cites W2056606865 @default.
• W2794440014 cites W2057872280 @default.
• W2794440014 cites W2059382856 @default.
• W2794440014 cites W2060718895 @default.
• W2794440014 cites W2063028611 @default.
• W2794440014 cites W2067029830 @default.
• W2794440014 cites W2072485260 @default.
• W2794440014 cites W2079904605 @default.
• W2794440014 cites W2080906705 @default.
• W2794440014 cites W2085457025 @default.
• W2794440014 cites W2099521715 @default.
• W2794440014 cites W2100055148 @default.
• W2794440014 cites W2102436900 @default.
• W2794440014 cites W2102888690 @default.
• W2794440014 cites W2103602723 @default.
• W2794440014 cites W2105796031 @default.
• W2794440014 cites W2107277218 @default.
• W2794440014 cites W2110944555 @default.
• W2794440014 cites W2113170201 @default.
• W2794440014 cites W2124144946 @default.
• W2794440014 cites W2125872855 @default.
• W2794440014 cites W2128828121 @default.
• W2794440014 cites W2131598474 @default.
• W2794440014 cites W2137683543 @default.
• W2794440014 cites W2142315987 @default.
• W2794440014 cites W2143109888 @default.
• W2794440014 cites W2147555013 @default.
• W2794440014 cites W2151785115 @default.
• W2794440014 cites W2154839974 @default.
• W2794440014 cites W2157513547 @default.
• W2794440014 cites W2166238981 @default.
• W2794440014 cites W2192080449 @default.
• W2794440014 cites W2220204085 @default.
• W2794440014 cites W2233790635 @default.
• W2794440014 cites W2238532141 @default.
• W2794440014 cites W2255671650 @default.
• W2794440014 cites W2277123861 @default.
• W2794440014 cites W2291301785 @default.
• W2794440014 cites W2317498500 @default.
• W2794440014 cites W2362156215 @default.
• W2794440014 cites W2397674976 @default.
• W2794440014 cites W2461668091 @default.
• W2794440014 cites W2498481263 @default.
• W2794440014 cites W2522187712 @default.
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• W2794440014 cites W2547148653 @default.
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• W2794440014 cites W2565996229 @default.
• W2794440014 cites W2598125375 @default.
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• W2794440014 cites W2613121768 @default.
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• W2794440014 doi "https://doi.org/10.1186/s13068-018-1018-y" @default.
• W2794440014 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5798184" @default.
• W2794440014 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29441126" @default.
• W2794440014 hasPublicationYear "2018" @default.
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