FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2794594747> ?p ?o ?g. }

• W2794594747 endingPage "364" @default.
• W2794594747 startingPage "359" @default.
• W2794594747 abstract "Helicobacter pylori is a gastric pathogen that is widely recognized as a causative agent of gastric disease. Its eradication is variable, mainly due to increased resistance to clarithromycin. Our objective was: to evaluate (i) if the biopsy specimen used for the rapid urease test is a useful sample to detect resistance to clarithromycin by PCR-RFLP and (ii) the distribution of A2142G and A2143G point mutations in the 23S rRNA gene, in relation to virulence factors in our region. Gastric specimens were collected from adult dyspeptic patients (n = 141) and H. pylori was investigated by the rapid urease test, histopathological analysis and PCR for the hsp60 gene. Clarithromycin resistance was detected by PCR-RFLP in 62 H. pylori (+) paired biopsy specimens submitted to molecular analysis and the rapid urease test. H. pylori virulence factors were analyzed by multiplex PCR using specific primers for the cagA, vacA and babA2 genes. Thirteen out of 62 strains (20.9%) were resistant to clarithromycin: 6/13 (46.2%) harbored the A2143G mutation whereas 7/13 (53.8%) carried the A2142G point mutation. vacA m1s1 was the most frequent genotype among the resistant strains. In conclusion, the biopsy specimens used for the rapid urease test were suitable samples for clarithromycin resistance detection in patients infected with H. pylori, which became especially useful in cases where the number or size of the biopsies is limited. In addition, this is the first report of a molecular analysis for clarithromycin resistance performed directly from gastric biopsies in our region. Helicobacter pylori es un patógeno ampliamente reconocido como causante de enfermedad gástrica. Su erradicación es variable, principalmente debido al incremento de la resistencia a claritromicina. Nuestros objetivos fueron evaluar la utilidad de la biopsia usada para realizar el test rápido de ureasa en la detección de resistencia a claritromicina por PCR-RFLP y conocer la distribución de las mutaciones puntuales A2142G y A2143G en el gen ARNr 23S, en relación con los factores de virulencia en nuestra región. Se recolectaron muestras gástricas (n=141) provenientes de pacientes adultos dispépticos y se investigó la presencia de H. pylori mediante el test rápido de ureasa, análisis histopatológico y PCR para el gen hsp60. La resistencia a claritromicina se analizó por PCR-RFLP en 62 muestras pareadas de biopsias gástricas H. pylori+ destinadas al análisis molecular y al test rápido de ureasa. Los factores de virulencia de H. pylori fueron analizados mediante PCR multiplex usando oligonucleótidos específicos para los genes cagA, vacA y babA2. Trece de 62 cepas (20,9%) fueron resistentes a claritromicina, 6/13 (46,2%) llevaron la mutación A2143G, mientras que 7/13 (53,8%) presentaron la mutación A2142G. El genotipo vacA s1m1 fue el más frecuente entre las cepas resistentes a claritromicina. En conclusión, las biopsias destinadas al test rápido de ureasa fueron muestras apropiadas para la detección de la resistencia a claritromicina en pacientes infectados con H. pylori. Esto es especialmente útil en aquellos casos en los que el número o el tamaño de las muestras son limitados. Además, este es el primer reporte de estudio de resistencia a claritromicina (mediante técnicas moleculares), directamente de biopsias gástricas en nuestra región." @default.
• W2794594747 created "2018-04-06" @default.
• W2794594747 creator A5020394750 @default.
• W2794594747 creator A5025759087 @default.
• W2794594747 creator A5029319738 @default.
• W2794594747 creator A5030950486 @default.
• W2794594747 creator A5040976876 @default.
• W2794594747 creator A5052357847 @default.
• W2794594747 creator A5069732649 @default.
• W2794594747 creator A5074510746 @default.
• W2794594747 creator A5086406240 @default.
• W2794594747 date "2018-10-01" @default.
• W2794594747 modified "2023-10-18" @default.
• W2794594747 title "Usefulness of rapid urease test samples for molecular analysis of clarithromycin resistance in Helicobacter pylori" @default.
• W2794594747 cites W1588993367 @default.
• W2794594747 cites W1930026458 @default.
• W2794594747 cites W1968884210 @default.
• W2794594747 cites W1972784340 @default.
• W2794594747 cites W1990896685 @default.
• W2794594747 cites W2002827185 @default.
• W2794594747 cites W2014499322 @default.
• W2794594747 cites W2018426253 @default.
• W2794594747 cites W2026849147 @default.
• W2794594747 cites W2030346571 @default.
• W2794594747 cites W2092677552 @default.
• W2794594747 cites W2098883254 @default.
• W2794594747 cites W2110367152 @default.
• W2794594747 cites W2119698116 @default.
• W2794594747 cites W2129309379 @default.
• W2794594747 cites W2135258395 @default.
• W2794594747 cites W2136210283 @default.
• W2794594747 cites W2136287933 @default.
• W2794594747 cites W2139742576 @default.
• W2794594747 cites W2143818784 @default.
• W2794594747 cites W2186049936 @default.
• W2794594747 cites W2314569332 @default.
• W2794594747 cites W2401076609 @default.
• W2794594747 cites W2461291790 @default.
• W2794594747 cites W2462499681 @default.
• W2794594747 cites W2464958356 @default.
• W2794594747 cites W2527848138 @default.
• W2794594747 doi "https://doi.org/10.1016/j.ram.2017.11.005" @default.
• W2794594747 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29602600" @default.
• W2794594747 hasPublicationYear "2018" @default.
• W2794594747 type Work @default.
• W2794594747 sameAs 2794594747 @default.
• W2794594747 citedByCount "6" @default.
• W2794594747 countsByYear W27945947472019 @default.
• W2794594747 countsByYear W27945947472021 @default.
• W2794594747 countsByYear W27945947472022 @default.
• W2794594747 countsByYear W27945947472023 @default.
• W2794594747 crossrefType "journal-article" @default.
• W2794594747 hasAuthorship W2794594747A5020394750 @default.
• W2794594747 hasAuthorship W2794594747A5025759087 @default.
• W2794594747 hasAuthorship W2794594747A5029319738 @default.
• W2794594747 hasAuthorship W2794594747A5030950486 @default.
• W2794594747 hasAuthorship W2794594747A5040976876 @default.
• W2794594747 hasAuthorship W2794594747A5052357847 @default.
• W2794594747 hasAuthorship W2794594747A5069732649 @default.
• W2794594747 hasAuthorship W2794594747A5074510746 @default.
• W2794594747 hasAuthorship W2794594747A5086406240 @default.
• W2794594747 hasBestOaLocation W27945947471 @default.
• W2794594747 hasConcept C104317684 @default.
• W2794594747 hasConcept C135763542 @default.
• W2794594747 hasConcept C176944494 @default.
• W2794594747 hasConcept C2776409635 @default.
• W2794594747 hasConcept C2776962512 @default.
• W2794594747 hasConcept C2778677798 @default.
• W2794594747 hasConcept C2780708398 @default.
• W2794594747 hasConcept C2780832600 @default.
• W2794594747 hasConcept C2910408639 @default.
• W2794594747 hasConcept C40749294 @default.
• W2794594747 hasConcept C49105822 @default.
• W2794594747 hasConcept C501734568 @default.
• W2794594747 hasConcept C54355233 @default.
• W2794594747 hasConcept C60987743 @default.
• W2794594747 hasConcept C67705224 @default.
• W2794594747 hasConcept C86803240 @default.
• W2794594747 hasConcept C88478588 @default.
• W2794594747 hasConcept C89423630 @default.
• W2794594747 hasConcept C90583042 @default.
• W2794594747 hasConceptScore W2794594747C104317684 @default.
• W2794594747 hasConceptScore W2794594747C135763542 @default.
• W2794594747 hasConceptScore W2794594747C176944494 @default.
• W2794594747 hasConceptScore W2794594747C2776409635 @default.
• W2794594747 hasConceptScore W2794594747C2776962512 @default.
• W2794594747 hasConceptScore W2794594747C2778677798 @default.
• W2794594747 hasConceptScore W2794594747C2780708398 @default.
• W2794594747 hasConceptScore W2794594747C2780832600 @default.
• W2794594747 hasConceptScore W2794594747C2910408639 @default.
• W2794594747 hasConceptScore W2794594747C40749294 @default.
• W2794594747 hasConceptScore W2794594747C49105822 @default.
• W2794594747 hasConceptScore W2794594747C501734568 @default.
• W2794594747 hasConceptScore W2794594747C54355233 @default.
• W2794594747 hasConceptScore W2794594747C60987743 @default.
• W2794594747 hasConceptScore W2794594747C67705224 @default.
• W2794594747 hasConceptScore W2794594747C86803240 @default.
• W2794594747 hasConceptScore W2794594747C88478588 @default.

• next