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- W2794635962 endingPage "e1007288" @default.
- W2794635962 startingPage "e1007288" @default.
- W2794635962 abstract "Modification defects in the tRNA anticodon loop often impair yeast growth and cause human disease. In the budding yeast Saccharomyces cerevisiae and the phylogenetically distant fission yeast Schizosaccharomyces pombe, trm7Δ mutants grow poorly due to lack of 2'-O-methylation of C32 and G34 in the tRNAPhe anticodon loop, and lesions in the human TRM7 homolog FTSJ1 cause non-syndromic X-linked intellectual disability (NSXLID). However, it is unclear why trm7Δ mutants grow poorly. We show here that despite the fact that S. cerevisiae trm7Δ mutants had no detectable tRNAPhe charging defect in rich media, the cells constitutively activated a robust general amino acid control (GAAC) response, acting through Gcn2, which senses uncharged tRNA. Consistent with reduced available charged tRNAPhe, the trm7Δ growth defect was suppressed by spontaneous mutations in phenylalanyl-tRNA synthetase (PheRS) or in the pol III negative regulator MAF1, and by overexpression of tRNAPhe, PheRS, or EF-1A; all of these also reduced GAAC activation. Genetic analysis also demonstrated that the trm7Δ growth defect was due to the constitutive robust GAAC activation as well as to the reduced available charged tRNAPhe. Robust GAAC activation was not observed with several other anticodon loop modification mutants. Analysis of S. pombe trm7 mutants led to similar observations. S. pombe Trm7 depletion also resulted in no observable tRNAPhe charging defect and a robust GAAC response, and suppressors mapped to PheRS and reduced GAAC activation. We speculate that GAAC activation is widely conserved in trm7 mutants in eukaryotes, including metazoans, and might play a role in FTSJ1-mediated NSXLID." @default.
- W2794635962 created "2018-04-06" @default.
- W2794635962 creator A5011365621 @default.
- W2794635962 creator A5015239643 @default.
- W2794635962 creator A5059864771 @default.
- W2794635962 creator A5075377059 @default.
- W2794635962 date "2018-03-29" @default.
- W2794635962 modified "2023-10-12" @default.
- W2794635962 title "Lack of 2'-O-methylation in the tRNA anticodon loop of two phylogenetically distant yeast species activates the general amino acid control pathway" @default.
- W2794635962 cites W1512753316 @default.
- W2794635962 cites W1528221774 @default.
- W2794635962 cites W1528770098 @default.
- W2794635962 cites W1561895274 @default.
- W2794635962 cites W1607969426 @default.
- W2794635962 cites W1659944386 @default.
- W2794635962 cites W1755978981 @default.
- W2794635962 cites W1917073860 @default.
- W2794635962 cites W1935366897 @default.
- W2794635962 cites W1974067623 @default.
- W2794635962 cites W1975474450 @default.
- W2794635962 cites W1979117468 @default.
- W2794635962 cites W1982749428 @default.
- W2794635962 cites W1988202801 @default.
- W2794635962 cites W1998845147 @default.
- W2794635962 cites W1999249940 @default.
- W2794635962 cites W2000928070 @default.
- W2794635962 cites W2000983517 @default.
- W2794635962 cites W2001890728 @default.
- W2794635962 cites W2002036062 @default.
- W2794635962 cites W2006178602 @default.
- W2794635962 cites W2007146973 @default.
- W2794635962 cites W2007783008 @default.
- W2794635962 cites W2007982521 @default.
- W2794635962 cites W2014194720 @default.
- W2794635962 cites W2017936435 @default.
- W2794635962 cites W2021406306 @default.
- W2794635962 cites W2022465064 @default.
- W2794635962 cites W2029083573 @default.
- W2794635962 cites W2040485185 @default.
- W2794635962 cites W2040531711 @default.
- W2794635962 cites W2043522659 @default.
- W2794635962 cites W2048284469 @default.
- W2794635962 cites W2050906087 @default.
- W2794635962 cites W2051409304 @default.
- W2794635962 cites W2051438073 @default.
- W2794635962 cites W2054811674 @default.
- W2794635962 cites W2056036930 @default.
- W2794635962 cites W2056381504 @default.
- W2794635962 cites W2059346861 @default.
- W2794635962 cites W2069089035 @default.
- W2794635962 cites W2069169076 @default.
- W2794635962 cites W2072811676 @default.
- W2794635962 cites W2075198363 @default.
- W2794635962 cites W2077008773 @default.
- W2794635962 cites W2077514275 @default.
- W2794635962 cites W2077997107 @default.
- W2794635962 cites W2081351825 @default.
- W2794635962 cites W2082125720 @default.
- W2794635962 cites W2084954024 @default.
- W2794635962 cites W2089004878 @default.
- W2794635962 cites W2090011988 @default.
- W2794635962 cites W2093035629 @default.
- W2794635962 cites W2097467142 @default.
- W2794635962 cites W2097843864 @default.
- W2794635962 cites W2097884184 @default.
- W2794635962 cites W2098257366 @default.
- W2794635962 cites W2099590260 @default.
- W2794635962 cites W2106114996 @default.
- W2794635962 cites W2106579945 @default.
- W2794635962 cites W2109535551 @default.
- W2794635962 cites W2110374270 @default.
- W2794635962 cites W2112836983 @default.
- W2794635962 cites W2113766528 @default.
- W2794635962 cites W2116289946 @default.
- W2794635962 cites W2116976668 @default.
- W2794635962 cites W2118397994 @default.
- W2794635962 cites W2119635233 @default.
- W2794635962 cites W2122500419 @default.
- W2794635962 cites W2122665705 @default.
- W2794635962 cites W2124924991 @default.
- W2794635962 cites W2127749120 @default.
- W2794635962 cites W2129282380 @default.
- W2794635962 cites W2132315340 @default.
- W2794635962 cites W2134224359 @default.
- W2794635962 cites W2139665070 @default.
- W2794635962 cites W2143280961 @default.
- W2794635962 cites W2144269180 @default.
- W2794635962 cites W2146494447 @default.
- W2794635962 cites W2153401759 @default.
- W2794635962 cites W2155937527 @default.
- W2794635962 cites W2158031669 @default.
- W2794635962 cites W2159696581 @default.
- W2794635962 cites W2162759883 @default.
- W2794635962 cites W2163736445 @default.
- W2794635962 cites W2236156346 @default.
- W2794635962 cites W2275510170 @default.
- W2794635962 cites W2313988137 @default.
- W2794635962 cites W2319304516 @default.