Matches in SemOpenAlex for { <https://semopenalex.org/work/W2794669082> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2794669082 abstract "Drymaria cordata is an important ethnomedicinal plant from which many important secondary metabolites have been reported. Partial purification of the enzyme, fructose 1,6-bisphosphatase was carried out following the methods of homogenization, streptomycin sulphate precipitation, ammonium sulphate cut and molecular sieve chromatography through Bio-Gel A-0.5m column. Biochemical characterization experiments were performed by standard methods with the enzyme preparation as purified from the column. Cytosolic fructose 1,6-bisphosphatase from the leaves of Drymaria cordata was purified to about 27-fold with 77% of recovery over homogenate fraction. The enzyme was highly specific to D-fructose-1,6-bisphosphate. With increase of protein concentration upto 300µg and incubation time upto120 minutes, the enzyme activity increased linearly. The metal ions Mg2+ or Mn2+ strongly stimulated the enzyme activity on the other hand Li+, Hg2+ and Zn2+ were potent inhibitors. The D. cordata enzyme showed temperature maxima at 40˚C while the optimum pH was at 8.0. The Km value of the enzyme for its substrate, Fructose 1,6-bisphosphate was 1.11µM proving its strong affinity." @default.
- W2794669082 created "2018-04-06" @default.
- W2794669082 creator A5007868834 @default.
- W2794669082 creator A5011212697 @default.
- W2794669082 creator A5056549517 @default.
- W2794669082 date "2018-03-31" @default.
- W2794669082 modified "2023-09-24" @default.
- W2794669082 title "Partial purification and characterisation of cytosolic Fructose-1, 6-bisphosphatase from Drymaria cordata" @default.
- W2794669082 doi "https://doi.org/10.21746/aps.2018.7.4.9" @default.
- W2794669082 hasPublicationYear "2018" @default.
- W2794669082 type Work @default.
- W2794669082 sameAs 2794669082 @default.
- W2794669082 citedByCount "0" @default.
- W2794669082 crossrefType "journal-article" @default.
- W2794669082 hasAuthorship W2794669082A5007868834 @default.
- W2794669082 hasAuthorship W2794669082A5011212697 @default.
- W2794669082 hasAuthorship W2794669082A5056549517 @default.
- W2794669082 hasBestOaLocation W27946690821 @default.
- W2794669082 hasConcept C130217890 @default.
- W2794669082 hasConcept C178790620 @default.
- W2794669082 hasConcept C179247698 @default.
- W2794669082 hasConcept C181199279 @default.
- W2794669082 hasConcept C185592680 @default.
- W2794669082 hasConcept C18903297 @default.
- W2794669082 hasConcept C189909181 @default.
- W2794669082 hasConcept C2776970464 @default.
- W2794669082 hasConcept C2778722038 @default.
- W2794669082 hasConcept C2779870022 @default.
- W2794669082 hasConcept C28212737 @default.
- W2794669082 hasConcept C43617362 @default.
- W2794669082 hasConcept C55493867 @default.
- W2794669082 hasConcept C86803240 @default.
- W2794669082 hasConcept C98539663 @default.
- W2794669082 hasConceptScore W2794669082C130217890 @default.
- W2794669082 hasConceptScore W2794669082C178790620 @default.
- W2794669082 hasConceptScore W2794669082C179247698 @default.
- W2794669082 hasConceptScore W2794669082C181199279 @default.
- W2794669082 hasConceptScore W2794669082C185592680 @default.
- W2794669082 hasConceptScore W2794669082C18903297 @default.
- W2794669082 hasConceptScore W2794669082C189909181 @default.
- W2794669082 hasConceptScore W2794669082C2776970464 @default.
- W2794669082 hasConceptScore W2794669082C2778722038 @default.
- W2794669082 hasConceptScore W2794669082C2779870022 @default.
- W2794669082 hasConceptScore W2794669082C28212737 @default.
- W2794669082 hasConceptScore W2794669082C43617362 @default.
- W2794669082 hasConceptScore W2794669082C55493867 @default.
- W2794669082 hasConceptScore W2794669082C86803240 @default.
- W2794669082 hasConceptScore W2794669082C98539663 @default.
- W2794669082 hasLocation W27946690821 @default.
- W2794669082 hasOpenAccess W2794669082 @default.
- W2794669082 hasPrimaryLocation W27946690821 @default.
- W2794669082 hasRelatedWork W1967948959 @default.
- W2794669082 hasRelatedWork W1994120895 @default.
- W2794669082 hasRelatedWork W199608434 @default.
- W2794669082 hasRelatedWork W2003746621 @default.
- W2794669082 hasRelatedWork W2042810599 @default.
- W2794669082 hasRelatedWork W2048294094 @default.
- W2794669082 hasRelatedWork W2051056043 @default.
- W2794669082 hasRelatedWork W2056966682 @default.
- W2794669082 hasRelatedWork W2082203946 @default.
- W2794669082 hasRelatedWork W2082239951 @default.
- W2794669082 hasRelatedWork W2199064410 @default.
- W2794669082 hasRelatedWork W2350549262 @default.
- W2794669082 hasRelatedWork W2356336969 @default.
- W2794669082 hasRelatedWork W2394988640 @default.
- W2794669082 hasRelatedWork W2415919108 @default.
- W2794669082 hasRelatedWork W2789613367 @default.
- W2794669082 hasRelatedWork W3021692933 @default.
- W2794669082 hasRelatedWork W3141799269 @default.
- W2794669082 hasRelatedWork W3209511036 @default.
- W2794669082 hasRelatedWork W2185286539 @default.
- W2794669082 isParatext "false" @default.
- W2794669082 isRetracted "false" @default.
- W2794669082 magId "2794669082" @default.
- W2794669082 workType "article" @default.