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- W2794678908 abstract "Urine samples are commonly analysed in cases with suspected illicit drug consumption. In events of alleged sample mishandling, urine sample source identification may be necessary. A simple DNA extraction procedure suitable for STR typing of urine samples was established on the Promega Maxwell® 16 paramagnetic silica bead platform. A small sample volume of 1.7 mL was used. Samples were stored at room temperature, 4 °C and −20 °C for 100 days to investigate the influence of storage temperature and time on extracted DNA quantity and success rate of STR typing. Samples stored at room temperature exhibited a faster decline in DNA yield with time and lower typing success rates as compared to those at 4 °C and −20 °C. This trend can likely be attributed to DNA degradation. In conclusion, this study presents a quick and effective DNA extraction protocol from a small urine volume stored for up to 100 days at 4 °C and −20 °C." @default.
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- W2794678908 date "2018-06-01" @default.
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- W2794678908 title "Simple DNA extraction of urine samples: Effects of storage temperature and storage time" @default.
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- W2794678908 doi "https://doi.org/10.1016/j.forsciint.2018.03.035" @default.
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