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• W2794688550 abstract "Leukocyte infiltration plays critical roles in tissue inflammation for pathogen clearance and tumor eradication. This process is regulated by complex microenvironments in blood vessels, including inflamed endothelium, blood flow, and perivascular components. The role of perivascular components in leukocyte infiltration has not been systematically investigated until recently mostly due to lack of technology. In this work, we developed a three-dimensional multi-layered blood vessel/tissue model with a nanofiber membrane, enabling real-time visualization of dynamic leukocyte infiltration and subsequent interaction with perivascular macrophages. We directly fabricated a highly aligned, free-standing nanofiber membrane with an ultra-thin thickness of ∼1 μm in microfluidic systems. Coating the nanofiber membrane with matrigel showed synergetic topographical and biochemical effects on the reconstitution of a well-aligned endothelial monolayer on the membrane. Our 3D multi-layered blood vessel/tissue model will offer a powerful and versatile tool for investigating the mechanism of leukocyte tissue infiltration and subsequent immune responses." @default.
• W2794688550 created "2018-04-06" @default.
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• W2794688550 date "2018-07-01" @default.
• W2794688550 modified "2023-10-12" @default.
• W2794688550 title "Ultra-thin, aligned, free-standing nanofiber membranes to recapitulate multi-layered blood vessel/tissue interface for leukocyte infiltration study" @default.
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• W2794688550 doi "https://doi.org/10.1016/j.biomaterials.2018.03.053" @default.
• W2794688550 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29631165" @default.
• W2794688550 hasPublicationYear "2018" @default.
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