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• W2795293810 abstract "ABSTRACT Cellular membranes feature dynamic submicrometer-scale lateral membrane domainsvariously referred to as lipid rafts, membrane rafts or glycosphingolipid-enriched microdomains (GEM). In order to understand the molecular functions of lipid rafts, numerous studies have utilized various biochemical methods to isolate and examine the protein composition of membrane rafts. However, interpretation of individual raft proteomics studies are confounded by the limitations of isolation methods and the dynamic nature of rafts. Knowledge-based approaches can facilitate biological data interpretation by integrating experimental evidence from existing studies. To this end, we previously developed RaftProt ( http://lipid-raft-database.di.uq.edu.au/ ), a searchable database of mammalian lipid raft-associated proteins. Despite being a valuable and highly used resource, improvements in search capabilities and visualisation were still needed. Here, we present RaftProt V2 ( http://raftprot.org ), an improved update of RaftProt, enabling interrogation and integration of datasets at the cell/tissue type and UniRef/Gene level. Besides the addition of new datasets and re-mapping of all entries to both UniProt and UniRef IDs, we have annotated the level of experimental evidence for each protein entry. The search engine now allows for multiple protein or experiment searches where correlations, interactions or overlaps can be investigated. The web-interface has been completely re-designed and offers new interactive tools for data and subset selection, correlation analysis and network visualization. Overall, RaftProt aims to advance our understanding of lipid raft function by revealing the proteomes and pathways that are associated with membrane microdomains in diverse tissue and conditions. Database URL: http://raftprot.org" @default.
• W2795293810 created "2018-04-06" @default.
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• W2795293810 date "2018-03-26" @default.
• W2795293810 modified "2023-09-26" @default.
• W2795293810 title "RaftProt V2: understanding membrane microdomain function through lipid raft proteomes" @default.
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• W2795293810 doi "https://doi.org/10.1101/286039" @default.
• W2795293810 hasPublicationYear "2018" @default.
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