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- W2795463158 abstract "We developed a rapid and robust HPLC-MS/MS (QqQ) method for the quantification of hepcidin-25, a promising new biomarker in iron metabolism, in human samples. The novelty of the method is the use of special HPLC vials to avoid adsorptive losses due to the basic character of the peptide that causes interaction with the silanol groups of the vial’s glass surface. Up to 90% decrease in the MS/MS signal was observed, when commercial HPLC vials were used, while vials treated with 3-(2-aminoethylamino)propylmethyldimethoxysilane or 1H,1H,2H,2H-perfluorooctyltriethoxysilane, leading to no losses in the range of physiological hepcidin-25 mean serum levels (10-20 µg/L)." @default.
- W2795463158 created "2018-04-13" @default.
- W2795463158 creator A5076031245 @default.
- W2795463158 date "2017-01-01" @default.
- W2795463158 modified "2023-09-26" @default.
- W2795463158 title "Troubleshooting for the clinical measurement of iron biomarkers using LC-MS/MS: Suppression of sample losses in autosampler vials" @default.
- W2795463158 hasPublicationYear "2017" @default.
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