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- W2795549649 abstract "Super-resolution fluoresce microscopy techniques break the diffraction limit in biological systems, promising a resolution ultimately limited only by the size of the emitting molecules. These methods, however, demand high numerical aperture (NA) lenses, in turn involving short working distances (typically below 1mm), while their imaging capability rapidly decays in turbid samples. Here we demonstrate that the unavoidable light scattering arising in biosystems can be exploited to generate sub-diffraction images using low NA and long working distances. In the Scattering-Assisted localiza- tion Microscopy (SAM), the resolution is defined by the grain size of the speckle pattern on a sample rather than by the collection optics. We report a four-fold increase in resolution compared to the theoretical diffraction limit, enabling, as an example, the detection of micron-sized Beta Amyloid plaques in brain tissues using a lens of 25 mm working distance and minimal laser power." @default.
- W2795549649 created "2018-04-13" @default.
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- W2795549649 date "2018-04-04" @default.
- W2795549649 modified "2023-09-27" @default.
- W2795549649 title "Scattering-assisted localization microscopy" @default.
- W2795549649 hasPublicationYear "2018" @default.
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