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- W2795720994 abstract "Abstract Intracellular deposits of α‐synuclein in the form of Lewy bodies are major hallmarks of Parkinson's disease (PD) and a range of related neurodegenerative disorders. Post‐translational modifications (PTMs) of α‐synuclein are increasingly thought to be major modulators of its structure, function, degradation and toxicity. Among these PTMs, phosphorylation near the C‐terminus at S129 has emerged as a dominant pathogenic modification as it is consistently observed to occur within the brain and cerebrospinal fluid (CSF) of post‐mortem PD patients, and its level appears to correlate with disease progression. Phosphorylation at the neighboring tyrosine residue Y125 has also been shown to protect against α‐synuclein toxicity in a Drosophila model of PD. In the present study we address the potential roles of C‐terminal phosphorylation in modulating the interaction of α‐synuclein with other protein partners, using a single domain antibody fragment (NbSyn87) that binds to the C‐terminal region of α‐synuclein with nanomolar affinity. The results reveal that phosphorylation at S129 has negligible effect on the binding affinity of NbSyn87 to α‐synuclein while phosphorylation at Y125, only four residues away, decreases the binding affinity by a factor of 400. These findings show that, despite the fact that α‐synuclein is intrinsically disordered in solution, selective phosphorylation can modulate significantly its interactions with other molecules and suggest how this particular form of modification could play a key role in regulating the normal and aberrant function of α‐synuclein." @default.
- W2795720994 created "2018-04-13" @default.
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- W2795720994 date "2018-07-01" @default.
- W2795720994 modified "2023-10-01" @default.
- W2795720994 title "Exploring the role of post-translational modifications in regulating α-synuclein interactions by studying the effects of phosphorylation on nanobody binding" @default.
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- W2795720994 doi "https://doi.org/10.1002/pro.3412" @default.
- W2795720994 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6032363" @default.
- W2795720994 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29603451" @default.
- W2795720994 hasPublicationYear "2018" @default.
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