FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2795741086> ?p ?o ?g. }

• W2795741086 endingPage "62" @default.
• W2795741086 startingPage "55" @default.
• W2795741086 abstract "γ-Glutamyltransferase (GGT) catalyzes the transfer of the γ-glutamyl moiety from a donor substrate such as glutathione to water (hydrolysis) or to an acceptor amino acid (transpeptidation) through the formation of a γ-glutamyl enzyme intermediate. The vast majority of the known GGTs has a short sequence covering the glutamate binding site, called lid-loop. Although being conserved enzymes, both B. subtilis GGT and the related enzyme CapD from B. anthracis lack the lid loop and, differently from other GGTs, both accept poly-γ-glutamic acid (γ-PGA) as a substrate. Starting from this observation, in this work the activity of an engineered mutant enzyme containing the amino acid sequence of the lid loop from E. coli GGT inserted into the backbone of B. subtilis GGT was compared to that of the lid loop-deficient B. subtilis GGT and the lid loop-carrier E. coli GGT. Results indicate that the absence of the lid loop seems not to be the sole structural feature responsible for the recognition of a polymeric substrate by GGTs. Nevertheless, time course of hydrolysis reactions carried out using oligo-γ-glutamyl glutamines as substrates showed that the lid loop acts as a gating structure, allowing the preferential selection of the small glutamine with respect to the oligomeric substrates. In this respect, the mutant B. subtilis GGT revealed to be more similar to E. coli GGT than to its wild-type counterpart. In addition, the transpeptidase activity of the newly produced mutant enzyme revealed to be higher with respect to that of both E. coli and wild-type B. subtilis GGT. These findings can be helpful in selecting GGTs intended as biocatalysts for preparative purposes as well as in designing mutant enzymes with improved transpeptidase activity." @default.
• W2795741086 created "2018-04-13" @default.
• W2795741086 creator A5003413432 @default.
• W2795741086 creator A5046802208 @default.
• W2795741086 creator A5047195845 @default.
• W2795741086 creator A5056931896 @default.
• W2795741086 creator A5078908847 @default.
• W2795741086 date "2018-07-01" @default.
• W2795741086 modified "2023-10-16" @default.
• W2795741086 title "Evidences on the role of the lid loop of γ-glutamyltransferases (GGT) in substrate selection" @default.
• W2795741086 cites W1578341309 @default.
• W2795741086 cites W1594945568 @default.
• W2795741086 cites W1633567371 @default.
• W2795741086 cites W1648994921 @default.
• W2795741086 cites W1827148040 @default.
• W2795741086 cites W1951830410 @default.
• W2795741086 cites W1966901153 @default.
• W2795741086 cites W1975505603 @default.
• W2795741086 cites W1984109103 @default.
• W2795741086 cites W1989259791 @default.
• W2795741086 cites W1992108973 @default.
• W2795741086 cites W2003078070 @default.
• W2795741086 cites W2006373747 @default.
• W2795741086 cites W2009336326 @default.
• W2795741086 cites W2015496910 @default.
• W2795741086 cites W2018320925 @default.
• W2795741086 cites W2020091120 @default.
• W2795741086 cites W2027697602 @default.
• W2795741086 cites W2031411119 @default.
• W2795741086 cites W2036395398 @default.
• W2795741086 cites W2042792520 @default.
• W2795741086 cites W2043643409 @default.
• W2795741086 cites W2043906385 @default.
• W2795741086 cites W2050675244 @default.
• W2795741086 cites W2053448808 @default.
• W2795741086 cites W2053715143 @default.
• W2795741086 cites W2059480981 @default.
• W2795741086 cites W2060601473 @default.
• W2795741086 cites W2063603254 @default.
• W2795741086 cites W2069446149 @default.
• W2795741086 cites W2078190866 @default.
• W2795741086 cites W2090792051 @default.
• W2795741086 cites W2095210131 @default.
• W2795741086 cites W2105263496 @default.
• W2795741086 cites W2105512129 @default.
• W2795741086 cites W2106246537 @default.
• W2795741086 cites W2106387893 @default.
• W2795741086 cites W2112432272 @default.
• W2795741086 cites W2117484859 @default.
• W2795741086 cites W2140865551 @default.
• W2795741086 cites W2149443281 @default.
• W2795741086 cites W2163029410 @default.
• W2795741086 cites W2415906399 @default.
• W2795741086 cites W2519492692 @default.
• W2795741086 cites W2569563513 @default.
• W2795741086 cites W2894871293 @default.
• W2795741086 cites W4241950997 @default.
• W2795741086 cites W770382081 @default.
• W2795741086 doi "https://doi.org/10.1016/j.enzmictec.2018.04.001" @default.
• W2795741086 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29685354" @default.
• W2795741086 hasPublicationYear "2018" @default.
• W2795741086 type Work @default.
• W2795741086 sameAs 2795741086 @default.
• W2795741086 citedByCount "18" @default.
• W2795741086 countsByYear W27957410862018 @default.
• W2795741086 countsByYear W27957410862019 @default.
• W2795741086 countsByYear W27957410862020 @default.
• W2795741086 countsByYear W27957410862021 @default.
• W2795741086 countsByYear W27957410862022 @default.
• W2795741086 countsByYear W27957410862023 @default.
• W2795741086 crossrefType "journal-article" @default.
• W2795741086 hasAuthorship W2795741086A5003413432 @default.
• W2795741086 hasAuthorship W2795741086A5046802208 @default.
• W2795741086 hasAuthorship W2795741086A5047195845 @default.
• W2795741086 hasAuthorship W2795741086A5056931896 @default.
• W2795741086 hasAuthorship W2795741086A5078908847 @default.
• W2795741086 hasBestOaLocation W27957410862 @default.
• W2795741086 hasConcept C104317684 @default.
• W2795741086 hasConcept C143065580 @default.
• W2795741086 hasConcept C181199279 @default.
• W2795741086 hasConcept C185592680 @default.
• W2795741086 hasConcept C18903297 @default.
• W2795741086 hasConcept C2777272437 @default.
• W2795741086 hasConcept C2777289219 @default.
• W2795741086 hasConcept C2779349466 @default.
• W2795741086 hasConcept C515207424 @default.
• W2795741086 hasConcept C523546767 @default.
• W2795741086 hasConcept C54355233 @default.
• W2795741086 hasConcept C547475151 @default.
• W2795741086 hasConcept C55493867 @default.
• W2795741086 hasConcept C71240020 @default.
• W2795741086 hasConcept C86803240 @default.
• W2795741086 hasConceptScore W2795741086C104317684 @default.
• W2795741086 hasConceptScore W2795741086C143065580 @default.
• W2795741086 hasConceptScore W2795741086C181199279 @default.
• W2795741086 hasConceptScore W2795741086C185592680 @default.
• W2795741086 hasConceptScore W2795741086C18903297 @default.
• W2795741086 hasConceptScore W2795741086C2777272437 @default.

• next