Matches in SemOpenAlex for { <https://semopenalex.org/work/W2795801799> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2795801799 endingPage "559" @default.
- W2795801799 startingPage "550" @default.
- W2795801799 abstract "N-Glycan analysis is routinely performed for biotherapeutic protein characterization. A recently introduced N-glycan analysis kit using RapiFluor-MS (RFMS) labeling provides time savings over reductive amination labeling methods while also providing enhanced fluorescence (FLR) and mass spectrometry (MS) responses. This article demonstrates the semiautomation of this kit using an Andrew Alliance pipetting robot that promises further gains in productivity. This robotic platform uses standard manual pipettors and an optically guided arm to facilitate the automation of manual procedures. The manual RFMS protocol includes two heating and cooling steps during protein denaturation and de-N-glycosylation. However, the current Andrew Alliance automated platform cannot move reaction tubes to and from different heating blocks. As a result, samples prepared using the automated procedure remain in a computer-controlled Peltier effect heating block, requiring reoptimization of denaturation and de-N-glycosylation temperatures. Using hydrophilic interaction liquid chromatography to monitor the RFMS-labeled glycan profiles, the authors demonstrated the reproducibility of the automated protocol with percent relative standard deviations (RSDs) of 9%-19% for the total area and 0.8%-20% for the relative areas of major and minor glycoforms. Overall, the automated platform presented here proves to be a convenient and reliable solution for N-glycan preparation and analysis." @default.
- W2795801799 created "2018-04-13" @default.
- W2795801799 creator A5041885042 @default.
- W2795801799 creator A5068446979 @default.
- W2795801799 creator A5069416379 @default.
- W2795801799 creator A5087420743 @default.
- W2795801799 date "2018-12-01" @default.
- W2795801799 modified "2023-10-14" @default.
- W2795801799 title "Automated Preparation of MS-Sensitive Fluorescently Labeled N-Glycans with a Commercial Pipetting Robot" @default.
- W2795801799 cites W1588728135 @default.
- W2795801799 cites W2087298314 @default.
- W2795801799 cites W2326601291 @default.
- W2795801799 cites W2330451514 @default.
- W2795801799 cites W2564001056 @default.
- W2795801799 cites W2574222163 @default.
- W2795801799 cites W2622632570 @default.
- W2795801799 doi "https://doi.org/10.1177/2472630318762384" @default.
- W2795801799 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6249649" @default.
- W2795801799 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29618278" @default.
- W2795801799 hasPublicationYear "2018" @default.
- W2795801799 type Work @default.
- W2795801799 sameAs 2795801799 @default.
- W2795801799 citedByCount "12" @default.
- W2795801799 countsByYear W27958017992018 @default.
- W2795801799 countsByYear W27958017992020 @default.
- W2795801799 countsByYear W27958017992021 @default.
- W2795801799 countsByYear W27958017992022 @default.
- W2795801799 countsByYear W27958017992023 @default.
- W2795801799 crossrefType "journal-article" @default.
- W2795801799 hasAuthorship W2795801799A5041885042 @default.
- W2795801799 hasAuthorship W2795801799A5068446979 @default.
- W2795801799 hasAuthorship W2795801799A5069416379 @default.
- W2795801799 hasAuthorship W2795801799A5087420743 @default.
- W2795801799 hasBestOaLocation W27958017991 @default.
- W2795801799 hasConcept C108625454 @default.
- W2795801799 hasConcept C115901376 @default.
- W2795801799 hasConcept C127413603 @default.
- W2795801799 hasConcept C147789679 @default.
- W2795801799 hasConcept C151552171 @default.
- W2795801799 hasConcept C161790260 @default.
- W2795801799 hasConcept C185592680 @default.
- W2795801799 hasConcept C206212055 @default.
- W2795801799 hasConcept C2777313579 @default.
- W2795801799 hasConcept C2779938440 @default.
- W2795801799 hasConcept C43617362 @default.
- W2795801799 hasConcept C55493867 @default.
- W2795801799 hasConcept C78519656 @default.
- W2795801799 hasConceptScore W2795801799C108625454 @default.
- W2795801799 hasConceptScore W2795801799C115901376 @default.
- W2795801799 hasConceptScore W2795801799C127413603 @default.
- W2795801799 hasConceptScore W2795801799C147789679 @default.
- W2795801799 hasConceptScore W2795801799C151552171 @default.
- W2795801799 hasConceptScore W2795801799C161790260 @default.
- W2795801799 hasConceptScore W2795801799C185592680 @default.
- W2795801799 hasConceptScore W2795801799C206212055 @default.
- W2795801799 hasConceptScore W2795801799C2777313579 @default.
- W2795801799 hasConceptScore W2795801799C2779938440 @default.
- W2795801799 hasConceptScore W2795801799C43617362 @default.
- W2795801799 hasConceptScore W2795801799C55493867 @default.
- W2795801799 hasConceptScore W2795801799C78519656 @default.
- W2795801799 hasIssue "6" @default.
- W2795801799 hasLocation W27958017991 @default.
- W2795801799 hasLocation W27958017992 @default.
- W2795801799 hasLocation W27958017993 @default.
- W2795801799 hasLocation W27958017994 @default.
- W2795801799 hasOpenAccess W2795801799 @default.
- W2795801799 hasPrimaryLocation W27958017991 @default.
- W2795801799 hasRelatedWork W1986697589 @default.
- W2795801799 hasRelatedWork W2015666528 @default.
- W2795801799 hasRelatedWork W2036365452 @default.
- W2795801799 hasRelatedWork W2614128742 @default.
- W2795801799 hasRelatedWork W2682783302 @default.
- W2795801799 hasRelatedWork W2795801799 @default.
- W2795801799 hasRelatedWork W2802601363 @default.
- W2795801799 hasRelatedWork W2931261440 @default.
- W2795801799 hasRelatedWork W4200230300 @default.
- W2795801799 hasRelatedWork W4292068905 @default.
- W2795801799 hasVolume "23" @default.
- W2795801799 isParatext "false" @default.
- W2795801799 isRetracted "false" @default.
- W2795801799 magId "2795801799" @default.
- W2795801799 workType "article" @default.