Matches in SemOpenAlex for { <https://semopenalex.org/work/W2796007072> ?p ?o ?g. }
- W2796007072 endingPage "347" @default.
- W2796007072 startingPage "331" @default.
- W2796007072 abstract "This chapter describes the advanced assembly methods that could be applied to combinatorial pathway libraries. The following assembly strategies are based on homologous recombination and DNA repair mechanisms: DNA assembler, Gibson assembly, and sequence and ligation-independent cloning (SLIC). Another family of advanced DNA assembly techniques has been developed via the implementation of the type IIS endonucleases such as BsaI, which cleave the DNA outside of their recognition sites. Combinatorial pathway library screening strategies are more efficient in the identification of an optimized pathway. The Gibson assembly method was applied to generate a large combinatorial library of promoters and enzymes and to optimize a multienzyme pathway in the absence of a high-throughput assay. A BioBrick-like assembly strategy was used in a combinatorial library of engineered ribosomal binding sites (RBSs). A randomized BioBrick strategy combines the power of Gibson assembly and the modularity of the BioBrick standards." @default.
- W2796007072 created "2018-04-13" @default.
- W2796007072 creator A5014465828 @default.
- W2796007072 creator A5024169462 @default.
- W2796007072 creator A5058609796 @default.
- W2796007072 date "2018-03-30" @default.
- W2796007072 modified "2023-10-14" @default.
- W2796007072 title "Applying Advanced <scp>DNA</scp> Assembly Methods to Generate Pathway Libraries" @default.
- W2796007072 cites W1498348219 @default.
- W2796007072 cites W1965740284 @default.
- W2796007072 cites W1967210731 @default.
- W2796007072 cites W1967901576 @default.
- W2796007072 cites W1968602937 @default.
- W2796007072 cites W1969350272 @default.
- W2796007072 cites W1970893992 @default.
- W2796007072 cites W1971439989 @default.
- W2796007072 cites W1979705207 @default.
- W2796007072 cites W1980012819 @default.
- W2796007072 cites W1981471795 @default.
- W2796007072 cites W2003677456 @default.
- W2796007072 cites W2005329081 @default.
- W2796007072 cites W2006337384 @default.
- W2796007072 cites W2008880969 @default.
- W2796007072 cites W2012396824 @default.
- W2796007072 cites W2016667239 @default.
- W2796007072 cites W2022186026 @default.
- W2796007072 cites W2028343312 @default.
- W2796007072 cites W2029943478 @default.
- W2796007072 cites W2030289459 @default.
- W2796007072 cites W2030433030 @default.
- W2796007072 cites W2034717680 @default.
- W2796007072 cites W2034846223 @default.
- W2796007072 cites W2037455839 @default.
- W2796007072 cites W2037630877 @default.
- W2796007072 cites W2041618640 @default.
- W2796007072 cites W2042650553 @default.
- W2796007072 cites W2047360542 @default.
- W2796007072 cites W2049541231 @default.
- W2796007072 cites W2073265884 @default.
- W2796007072 cites W2079347688 @default.
- W2796007072 cites W2081799587 @default.
- W2796007072 cites W2089546919 @default.
- W2796007072 cites W2091731123 @default.
- W2796007072 cites W2102440675 @default.
- W2796007072 cites W2107523606 @default.
- W2796007072 cites W2112570909 @default.
- W2796007072 cites W2113983551 @default.
- W2796007072 cites W2115519265 @default.
- W2796007072 cites W2117179092 @default.
- W2796007072 cites W2119179068 @default.
- W2796007072 cites W2128809824 @default.
- W2796007072 cites W2134783465 @default.
- W2796007072 cites W2136536485 @default.
- W2796007072 cites W2144779133 @default.
- W2796007072 cites W2146372898 @default.
- W2796007072 cites W2150665661 @default.
- W2796007072 cites W2154797402 @default.
- W2796007072 cites W2160253662 @default.
- W2796007072 cites W2163568770 @default.
- W2796007072 cites W2170764254 @default.
- W2796007072 cites W2321143980 @default.
- W2796007072 doi "https://doi.org/10.1002/9783527688104.ch16" @default.
- W2796007072 hasPublicationYear "2018" @default.
- W2796007072 type Work @default.
- W2796007072 sameAs 2796007072 @default.
- W2796007072 citedByCount "1" @default.
- W2796007072 countsByYear W27960070722020 @default.
- W2796007072 crossrefType "other" @default.
- W2796007072 hasAuthorship W2796007072A5014465828 @default.
- W2796007072 hasAuthorship W2796007072A5024169462 @default.
- W2796007072 hasAuthorship W2796007072A5058609796 @default.
- W2796007072 hasBestOaLocation W27960070721 @default.
- W2796007072 hasConcept C102744134 @default.
- W2796007072 hasConcept C104317684 @default.
- W2796007072 hasConcept C121050878 @default.
- W2796007072 hasConcept C13575015 @default.
- W2796007072 hasConcept C150194340 @default.
- W2796007072 hasConcept C162317418 @default.
- W2796007072 hasConcept C18949551 @default.
- W2796007072 hasConcept C19014433 @default.
- W2796007072 hasConcept C191908910 @default.
- W2796007072 hasConcept C199360897 @default.
- W2796007072 hasConcept C2779478453 @default.
- W2796007072 hasConcept C3017666073 @default.
- W2796007072 hasConcept C41008148 @default.
- W2796007072 hasConcept C54355233 @default.
- W2796007072 hasConcept C552990157 @default.
- W2796007072 hasConcept C70721500 @default.
- W2796007072 hasConcept C86803240 @default.
- W2796007072 hasConceptScore W2796007072C102744134 @default.
- W2796007072 hasConceptScore W2796007072C104317684 @default.
- W2796007072 hasConceptScore W2796007072C121050878 @default.
- W2796007072 hasConceptScore W2796007072C13575015 @default.
- W2796007072 hasConceptScore W2796007072C150194340 @default.
- W2796007072 hasConceptScore W2796007072C162317418 @default.
- W2796007072 hasConceptScore W2796007072C18949551 @default.
- W2796007072 hasConceptScore W2796007072C19014433 @default.
- W2796007072 hasConceptScore W2796007072C191908910 @default.