Matches in SemOpenAlex for { <https://semopenalex.org/work/W2796274295> ?p ?o ?g. }
- W2796274295 endingPage "101" @default.
- W2796274295 startingPage "94" @default.
- W2796274295 abstract "Cryo-Electron Microscopy (cryo-EM) has become an invaluable tool for structural biology. Over the past decade, the advent of direct electron detectors and automated data acquisition has established cryo-EM as a central method in structural biology. However, challenges remain in the reliable and efficient preparation of samples in a manner which is compatible with high time resolution. The delivery of sample onto the grid is recognized as a critical step in the workflow as it is a source of variability and loss of material due to the blotting which is usually required. Here, we present a method for sample delivery and plunge freezing based on the use of Surface Acoustic Waves to deploy 6-8 µm droplets to the EM grid. This method minimises the sample dead volume and ensures vitrification within 52.6 ms from the moment the sample leaves the microfluidics chip. We demonstrate a working protocol to minimize the atomised volume and apply it to plunge freeze three different samples and provide proof that no damage occurs due to the interaction between the sample and the acoustic waves." @default.
- W2796274295 created "2018-04-13" @default.
- W2796274295 creator A5016136743 @default.
- W2796274295 creator A5019996250 @default.
- W2796274295 creator A5022727654 @default.
- W2796274295 creator A5028900913 @default.
- W2796274295 creator A5065089551 @default.
- W2796274295 creator A5076804968 @default.
- W2796274295 creator A5078068180 @default.
- W2796274295 date "2018-08-01" @default.
- W2796274295 modified "2023-10-01" @default.
- W2796274295 title "Delivery of femtolitre droplets using surface acoustic wave based atomisation for cryo-EM grid preparation" @default.
- W2796274295 cites W1503194715 @default.
- W2796274295 cites W1539491410 @default.
- W2796274295 cites W1635604571 @default.
- W2796274295 cites W1794395616 @default.
- W2796274295 cites W1851479635 @default.
- W2796274295 cites W1902980404 @default.
- W2796274295 cites W1986991256 @default.
- W2796274295 cites W1989601752 @default.
- W2796274295 cites W1995452275 @default.
- W2796274295 cites W1995713687 @default.
- W2796274295 cites W2013077656 @default.
- W2796274295 cites W2031907861 @default.
- W2796274295 cites W2039117718 @default.
- W2796274295 cites W2041956358 @default.
- W2796274295 cites W2048939228 @default.
- W2796274295 cites W2051143771 @default.
- W2796274295 cites W2052756790 @default.
- W2796274295 cites W2066934245 @default.
- W2796274295 cites W2070900621 @default.
- W2796274295 cites W2074789706 @default.
- W2796274295 cites W2084537898 @default.
- W2796274295 cites W2086818038 @default.
- W2796274295 cites W2087527280 @default.
- W2796274295 cites W2088612475 @default.
- W2796274295 cites W2103972113 @default.
- W2796274295 cites W2105976126 @default.
- W2796274295 cites W2111398700 @default.
- W2796274295 cites W2141768493 @default.
- W2796274295 cites W2149360962 @default.
- W2796274295 cites W2165429242 @default.
- W2796274295 cites W2168046582 @default.
- W2796274295 cites W2182347107 @default.
- W2796274295 cites W2261036342 @default.
- W2796274295 cites W2284964827 @default.
- W2796274295 cites W2292743908 @default.
- W2796274295 cites W2341655728 @default.
- W2796274295 cites W2409722811 @default.
- W2796274295 cites W2500177674 @default.
- W2796274295 cites W2516560469 @default.
- W2796274295 cites W2526539124 @default.
- W2796274295 cites W2551620402 @default.
- W2796274295 cites W2552374053 @default.
- W2796274295 cites W2587625522 @default.
- W2796274295 cites W2622393395 @default.
- W2796274295 cites W2624766684 @default.
- W2796274295 cites W2755567304 @default.
- W2796274295 cites W2950782282 @default.
- W2796274295 cites W2953320394 @default.
- W2796274295 doi "https://doi.org/10.1016/j.jsb.2018.03.012" @default.
- W2796274295 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29630922" @default.
- W2796274295 hasPublicationYear "2018" @default.
- W2796274295 type Work @default.
- W2796274295 sameAs 2796274295 @default.
- W2796274295 citedByCount "36" @default.
- W2796274295 countsByYear W27962742952018 @default.
- W2796274295 countsByYear W27962742952019 @default.
- W2796274295 countsByYear W27962742952020 @default.
- W2796274295 countsByYear W27962742952021 @default.
- W2796274295 countsByYear W27962742952022 @default.
- W2796274295 countsByYear W27962742952023 @default.
- W2796274295 crossrefType "journal-article" @default.
- W2796274295 hasAuthorship W2796274295A5016136743 @default.
- W2796274295 hasAuthorship W2796274295A5019996250 @default.
- W2796274295 hasAuthorship W2796274295A5022727654 @default.
- W2796274295 hasAuthorship W2796274295A5028900913 @default.
- W2796274295 hasAuthorship W2796274295A5065089551 @default.
- W2796274295 hasAuthorship W2796274295A5076804968 @default.
- W2796274295 hasAuthorship W2796274295A5078068180 @default.
- W2796274295 hasConcept C121332964 @default.
- W2796274295 hasConcept C171250308 @default.
- W2796274295 hasConcept C177212765 @default.
- W2796274295 hasConcept C185592680 @default.
- W2796274295 hasConcept C187691185 @default.
- W2796274295 hasConcept C192562407 @default.
- W2796274295 hasConcept C198531522 @default.
- W2796274295 hasConcept C20556612 @default.
- W2796274295 hasConcept C20702342 @default.
- W2796274295 hasConcept C2524010 @default.
- W2796274295 hasConcept C2778576202 @default.
- W2796274295 hasConcept C33923547 @default.
- W2796274295 hasConcept C41008148 @default.
- W2796274295 hasConcept C43617362 @default.
- W2796274295 hasConcept C55493867 @default.
- W2796274295 hasConcept C62520636 @default.
- W2796274295 hasConcept C77088390 @default.
- W2796274295 hasConcept C8673954 @default.