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- W2796452009 abstract "ABSCTRACT Although pooled CRISPR libraries are widely used in high-throughput screening to study various biological processes, library construction for researcher’s own study is a time-consuming, labor-intensive, and expensive process. In this study, we develop a simple, scalable method, called Cas-CLIP, to customize conventional pooled CRISPR libraries using the CRISPR/Cas9 system. We show that conventional pooled CRISPR libraries can be modified by eliminating gRNAs that target positive genes, enabling the identification of unknown target genes in CRISPR screening. Cas-CLIP is a precise method for customizing conventional pooled CRISPR libraries and will broaden the scope of high-throughput screening technology." @default.
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- W2796452009 date "2018-02-25" @default.
- W2796452009 modified "2023-09-27" @default.
- W2796452009 title "Cas-CLIP: a method for customizing pooled CRISPR libraries" @default.
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- W2796452009 doi "https://doi.org/10.1101/187757" @default.
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