Matches in SemOpenAlex for { <https://semopenalex.org/work/W2796626415> ?p ?o ?g. }
- W2796626415 endingPage "626" @default.
- W2796626415 startingPage "614" @default.
- W2796626415 abstract "Xenobiotic phthalates are industrially produced on the annual million ton scale. The oxygen-independent enzymatic reactions involved in anaerobic phthalate degradation have only recently been elucidated. In vitro assays suggested that phthalate is first activated to phthaloyl-CoA followed by decarboxylation to benzoyl-CoA. Here, we report the heterologous production and characterization of the enzyme initiating anaerobic phthalate degradation from 'Aromatoleum aromaticum': a highly specific succinyl-CoA:phthalate CoA transferase (SPT, class III CoA transferase). Phthaloyl-CoA formed by SPT accumulated only to sub-micromolar concentrations due to the extreme lability of the product towards intramolecular substitution with a half-life of around 7 min. Upon addition of excess phthaloyl-CoA decarboxylase (PCD), the combined activity of both enzymes was drastically shifted towards physiologically relevant benzoyl-CoA formation. In conclusion, a massive overproduction of PCD in phthalate-grown cells to concentrations >140 μM was observed that allowed for efficient phthaloyl-CoA conversion at concentrations 250-fold below the apparent Km -value of PCD. The results obtained provide insights into an only recently evolved xenobiotic degradation pathway where a massive cellular overproduction of PCD compensates for the formation of the probably most unstable CoA ester intermediate in biology." @default.
- W2796626415 created "2018-04-24" @default.
- W2796626415 creator A5029495036 @default.
- W2796626415 creator A5049778197 @default.
- W2796626415 creator A5077445480 @default.
- W2796626415 date "2018-04-26" @default.
- W2796626415 modified "2023-10-15" @default.
- W2796626415 title "Evolution of a xenobiotic degradation pathway: formation and capture of the labile phthaloyl-CoA intermediate during anaerobic phthalate degradation" @default.
- W2796626415 cites W1480704794 @default.
- W2796626415 cites W1485645868 @default.
- W2796626415 cites W1496602812 @default.
- W2796626415 cites W1498997676 @default.
- W2796626415 cites W1506317292 @default.
- W2796626415 cites W1518940859 @default.
- W2796626415 cites W1526671183 @default.
- W2796626415 cites W1597182881 @default.
- W2796626415 cites W1784890727 @default.
- W2796626415 cites W1829522902 @default.
- W2796626415 cites W1861776684 @default.
- W2796626415 cites W1970706674 @default.
- W2796626415 cites W1976331398 @default.
- W2796626415 cites W1985808740 @default.
- W2796626415 cites W1986234144 @default.
- W2796626415 cites W1986543704 @default.
- W2796626415 cites W1986887325 @default.
- W2796626415 cites W1991071919 @default.
- W2796626415 cites W1991149146 @default.
- W2796626415 cites W1999031366 @default.
- W2796626415 cites W1999994029 @default.
- W2796626415 cites W2006716299 @default.
- W2796626415 cites W2008323358 @default.
- W2796626415 cites W2019005194 @default.
- W2796626415 cites W2023316418 @default.
- W2796626415 cites W2026743918 @default.
- W2796626415 cites W2029433435 @default.
- W2796626415 cites W2032018766 @default.
- W2796626415 cites W2035644151 @default.
- W2796626415 cites W2048153230 @default.
- W2796626415 cites W2048687101 @default.
- W2796626415 cites W2048834746 @default.
- W2796626415 cites W2055043387 @default.
- W2796626415 cites W2058594842 @default.
- W2796626415 cites W2065756478 @default.
- W2796626415 cites W2066228976 @default.
- W2796626415 cites W2078643490 @default.
- W2796626415 cites W2098764106 @default.
- W2796626415 cites W2114902713 @default.
- W2796626415 cites W2127322768 @default.
- W2796626415 cites W2152207030 @default.
- W2796626415 cites W2155277410 @default.
- W2796626415 cites W2312520110 @default.
- W2796626415 cites W2346274355 @default.
- W2796626415 cites W2460308434 @default.
- W2796626415 cites W2460670816 @default.
- W2796626415 cites W2741831427 @default.
- W2796626415 cites W2790748531 @default.
- W2796626415 cites W4239740335 @default.
- W2796626415 cites W4255329491 @default.
- W2796626415 cites W4293247451 @default.
- W2796626415 cites W4297770494 @default.
- W2796626415 doi "https://doi.org/10.1111/mmi.13962" @default.
- W2796626415 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29645305" @default.
- W2796626415 hasPublicationYear "2018" @default.
- W2796626415 type Work @default.
- W2796626415 sameAs 2796626415 @default.
- W2796626415 citedByCount "15" @default.
- W2796626415 countsByYear W27966264152018 @default.
- W2796626415 countsByYear W27966264152019 @default.
- W2796626415 countsByYear W27966264152020 @default.
- W2796626415 countsByYear W27966264152021 @default.
- W2796626415 countsByYear W27966264152022 @default.
- W2796626415 countsByYear W27966264152023 @default.
- W2796626415 crossrefType "journal-article" @default.
- W2796626415 hasAuthorship W2796626415A5029495036 @default.
- W2796626415 hasAuthorship W2796626415A5049778197 @default.
- W2796626415 hasAuthorship W2796626415A5077445480 @default.
- W2796626415 hasBestOaLocation W27966264151 @default.
- W2796626415 hasConcept C115448650 @default.
- W2796626415 hasConcept C149997192 @default.
- W2796626415 hasConcept C161790260 @default.
- W2796626415 hasConcept C178790620 @default.
- W2796626415 hasConcept C181199279 @default.
- W2796626415 hasConcept C185592680 @default.
- W2796626415 hasConcept C2779679103 @default.
- W2796626415 hasConcept C2780595602 @default.
- W2796626415 hasConcept C2781005124 @default.
- W2796626415 hasConcept C41008148 @default.
- W2796626415 hasConcept C55493867 @default.
- W2796626415 hasConcept C76155785 @default.
- W2796626415 hasConcept C86803240 @default.
- W2796626415 hasConceptScore W2796626415C115448650 @default.
- W2796626415 hasConceptScore W2796626415C149997192 @default.
- W2796626415 hasConceptScore W2796626415C161790260 @default.
- W2796626415 hasConceptScore W2796626415C178790620 @default.
- W2796626415 hasConceptScore W2796626415C181199279 @default.
- W2796626415 hasConceptScore W2796626415C185592680 @default.
- W2796626415 hasConceptScore W2796626415C2779679103 @default.
- W2796626415 hasConceptScore W2796626415C2780595602 @default.