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- W2797084472 abstract "The occurrence of numerous structural isomers in glycans from biological sources presents a severe challenge for structural glycomics. The subtle differences among isomeric structures demand analytical methods that can provide structural details while working efficiently with on-line glycan separation methods. Although liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a powerful tool for mixture analysis, the commonly utilized collision-induced dissociation (CID) method often does not generate a sufficient number of fragments at the MS2 level for comprehensive structural characterization. Here, we studied the electronic excitation dissociation (EED) behaviors of metal-adducted, permethylated glycans, and identified key spectral features that could facilitate both topology and linkage determinations. We developed an EED-based, nanoscale, reversed phase (RP)LC-MS/MS platform, and demonstrated its ability to achieve complete structural elucidation of up to five structural isomers in a single LC-MS/MS analysis. Graphical Abstract." @default.
- W2797084472 created "2018-04-24" @default.
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- W2797084472 creator A5081519721 @default.
- W2797084472 creator A5091186437 @default.
- W2797084472 date "2018-04-13" @default.
- W2797084472 modified "2023-10-15" @default.
- W2797084472 title "Characterization of Isomeric Glycans by Reversed Phase Liquid Chromatography-Electronic Excitation Dissociation Tandem Mass Spectrometry" @default.
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- W2797084472 doi "https://doi.org/10.1007/s13361-018-1943-9" @default.
- W2797084472 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6004250" @default.
- W2797084472 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29654534" @default.
- W2797084472 hasPublicationYear "2018" @default.
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