FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2797539471> ?p ?o ?g. }

• W2797539471 endingPage "e0195880" @default.
• W2797539471 startingPage "e0195880" @default.
• W2797539471 abstract "Often Escherichia coli are harmless and/or beneficial bacteria inhabiting the gastrointestinal tract of livestock and humans. However, Shiga toxin-producing E. coli (STEC) have been linked to human disease. Cattle are the primary reservoir for STEC and STEC super-shedders are considered to be a major contributor in animal to animal transmission. Among STEC, O157:H7 is the most recognized serotype, but in recent years, non-O157 STEC have been increasingly linked to human disease. In Argentina and Germany, O178 is considered an emerging pathogen. Our objective was to compare populations of E. coli O178, O157, shiga toxin 1 and 2 in western Canadian cattle feces from a sampling pool of ~80,000 beef cattle collected at two slaughterhouses. Conventional PCR was utilized to screen 1,773 samples for presence/absence of E. coli O178. A subset of samples (n = 168) was enumerated using droplet digital PCR (ddPCR) and proportions of O178, O157 and shiga toxins 1 & 2 specific-fragments were calculated as a proportion of generic E. coli (GEC) specific-fragments. Distribution of stx1 and stx2 was determined by comparing stx1, stx2 and O157 enumerations. Conventional PCR detected the presence of O178 in 873 of 1,773 samples and ddPCR found the average proportion of O178, O157, stx1 and stx2 in the samples 2.8%, 0.6%, 1.4% and 0.5%, respectively. Quantification of stx1 and stx2 revealed more virulence genes than could be exclusively attributed to O157. Our results confirmed the presence of E. coli O178 in western Canadian cattle and ddPCR revealed O178 as a greater proportion of GEC than was O157. Our results suggests: I) O178 may be an emerging subgroup in Canada and II) monitoring virulence genes may be a more relevant target for food-safety STEC surveillance compared to current serogroup screening." @default.
• W2797539471 created "2018-04-24" @default.
• W2797539471 creator A5041029632 @default.
• W2797539471 creator A5043430996 @default.
• W2797539471 creator A5059014667 @default.
• W2797539471 creator A5062536994 @default.
• W2797539471 date "2018-04-12" @default.
• W2797539471 modified "2023-09-24" @default.
• W2797539471 title "Quantitative surveillance of shiga toxins 1 and 2, Escherichia coli O178 and O157 in feces of western-Canadian slaughter cattle enumerated by droplet digital PCR with a focus on seasonality and slaughterhouse location" @default.
• W2797539471 cites W1425628084 @default.
• W2797539471 cites W1521295680 @default.
• W2797539471 cites W1571086457 @default.
• W2797539471 cites W1698401339 @default.
• W2797539471 cites W1971533448 @default.
• W2797539471 cites W1982379136 @default.
• W2797539471 cites W2002027086 @default.
• W2797539471 cites W2011969644 @default.
• W2797539471 cites W2018146727 @default.
• W2797539471 cites W2028081076 @default.
• W2797539471 cites W2047255446 @default.
• W2797539471 cites W2070590269 @default.
• W2797539471 cites W2073512043 @default.
• W2797539471 cites W2074615576 @default.
• W2797539471 cites W2081805489 @default.
• W2797539471 cites W2092023357 @default.
• W2797539471 cites W2094448747 @default.
• W2797539471 cites W2094730822 @default.
• W2797539471 cites W2102050780 @default.
• W2797539471 cites W2102274776 @default.
• W2797539471 cites W2106646376 @default.
• W2797539471 cites W2111704428 @default.
• W2797539471 cites W2120368857 @default.
• W2797539471 cites W2125774503 @default.
• W2797539471 cites W2145545538 @default.
• W2797539471 cites W2165619858 @default.
• W2797539471 cites W2168446646 @default.
• W2797539471 cites W2170851600 @default.
• W2797539471 cites W2171679146 @default.
• W2797539471 cites W2315916323 @default.
• W2797539471 cites W2345099152 @default.
• W2797539471 cites W2395860034 @default.
• W2797539471 cites W2396857584 @default.
• W2797539471 cites W2402492340 @default.
• W2797539471 cites W2466097969 @default.
• W2797539471 cites W2477388835 @default.
• W2797539471 cites W2479547154 @default.
• W2797539471 cites W2538294284 @default.
• W2797539471 cites W2554303433 @default.
• W2797539471 cites W2606614280 @default.
• W2797539471 cites W2608670194 @default.
• W2797539471 cites W287057585 @default.
• W2797539471 cites W756441888 @default.
• W2797539471 cites W836888654 @default.
• W2797539471 doi "https://doi.org/10.1371/journal.pone.0195880" @default.
• W2797539471 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5897018" @default.
• W2797539471 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29649278" @default.
• W2797539471 hasPublicationYear "2018" @default.
• W2797539471 type Work @default.
• W2797539471 sameAs 2797539471 @default.
• W2797539471 citedByCount "8" @default.
• W2797539471 countsByYear W27975394712019 @default.
• W2797539471 countsByYear W27975394712020 @default.
• W2797539471 countsByYear W27975394712021 @default.
• W2797539471 countsByYear W27975394712022 @default.
• W2797539471 crossrefType "journal-article" @default.
• W2797539471 hasAuthorship W2797539471A5041029632 @default.
• W2797539471 hasAuthorship W2797539471A5043430996 @default.
• W2797539471 hasAuthorship W2797539471A5059014667 @default.
• W2797539471 hasAuthorship W2797539471A5062536994 @default.
• W2797539471 hasBestOaLocation W27975394711 @default.
• W2797539471 hasConcept C10389963 @default.
• W2797539471 hasConcept C104317684 @default.
• W2797539471 hasConcept C2776460866 @default.
• W2797539471 hasConcept C2778411228 @default.
• W2797539471 hasConcept C2781001907 @default.
• W2797539471 hasConcept C48126324 @default.
• W2797539471 hasConcept C49105822 @default.
• W2797539471 hasConcept C54355233 @default.
• W2797539471 hasConcept C547475151 @default.
• W2797539471 hasConcept C60987743 @default.
• W2797539471 hasConcept C61716771 @default.
• W2797539471 hasConcept C86803240 @default.
• W2797539471 hasConcept C89423630 @default.
• W2797539471 hasConceptScore W2797539471C10389963 @default.
• W2797539471 hasConceptScore W2797539471C104317684 @default.
• W2797539471 hasConceptScore W2797539471C2776460866 @default.
• W2797539471 hasConceptScore W2797539471C2778411228 @default.
• W2797539471 hasConceptScore W2797539471C2781001907 @default.
• W2797539471 hasConceptScore W2797539471C48126324 @default.
• W2797539471 hasConceptScore W2797539471C49105822 @default.
• W2797539471 hasConceptScore W2797539471C54355233 @default.
• W2797539471 hasConceptScore W2797539471C547475151 @default.
• W2797539471 hasConceptScore W2797539471C60987743 @default.
• W2797539471 hasConceptScore W2797539471C61716771 @default.
• W2797539471 hasConceptScore W2797539471C86803240 @default.
• W2797539471 hasConceptScore W2797539471C89423630 @default.
• W2797539471 hasIssue "4" @default.
• W2797539471 hasLocation W27975394711 @default.

• next