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- W2797635331 abstract "The brown planthopper ATP-binding cassette (ABC) proteins White (W), Scarlet (St) and Brown (Bw) belong to the ABC transporter superfamily and are responsible for the transportation of guanine and tryptophan precursors of eye pigments. In the present study, the brown planthopper White (NlW), S t(NlSt) and Bw (NlBw) genes were cloned, and subsequent phylogenetic analysis showed that these genes are clustered with their respective homologues, with a genetic relationship observed between NlW and its Bemisia tabaci homologue having the highest similarity. Sequence alignments showed that these three proteins have a highly conserved Walker A domain, an ABC “signature sequence” and a Walker B domain. QRT-PCR demonstrated that W, St and Bw are highly expressed in the head of long-winged males and are highly expressed in both egg and male. Adult eye colour was altered after the downregulation of NlW, NlSt and NlBw in the 1st to 3rd instar nymph. The eye colours of emerged adults became white, dark and red after injection of dsNlW, dsNlSt and dsNlBw, respectively. The eye pigment content assay revealed that xanthommatin and pteridine were significantly decreased after the injection of dsRNAs, and the range of variation was inversely correlated with nymph age. The present study provides a theoretical basis for understanding the function of ABC transporters at the molecular and biochemical levels." @default.
- W2797635331 created "2018-04-24" @default.
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- W2797635331 date "2018-07-01" @default.
- W2797635331 modified "2023-10-16" @default.
- W2797635331 title "Role of ABC transporters White , Scarlet and Brown in brown planthopper eye pigmentation" @default.
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- W2797635331 doi "https://doi.org/10.1016/j.cbpb.2018.04.003" @default.
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