FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2797783923> ?p ?o ?g. }

• W2797783923 endingPage "1578" @default.
• W2797783923 startingPage "1563" @default.
• W2797783923 abstract "Elastin-like proteins (ELPs) are known to undergo liquid-liquid phase separation reversibly above a concentration-dependent transition temperature. Previous studies suggested that, as temperature increases, ELPs experience an increased propensity for type II β-turns. However, how the ELPs behave below the phase transition temperature itself is still elusive. Here, we investigate the importance of β-turn formation during the early stages of ELP self-association. We examined the behavior of two ELPs, a 150-repeat construct that had been investigated previously (ELP[V5G3A2-150] as well as a new 40-repeat construct (ELP40) suitable for nuclear magnetic resonance measurements. Structural analysis of ELP40 reveals a disordered conformation, and chemical shifts throughout the sequence are insensitive to changes in temperature over 20°C. However, a low population of β-turn conformation cannot be ruled out based on chemical shifts alone. To examine the structural consequences of β-turns in ELPs, a series of structural ensembles of ELP[V5G3A2-150] were generated, incorporating differing amounts of β-turn bias throughout the chain. To mimic the early stages of the phase change, two monomers were paired, assuming preferential interaction at β-turn regions. This approach was justified by the observation that buried hydrophobic turns are commonly observed to interact in the Protein Data Bank. After dimerization, the ensemble-averaged hydrodynamic properties were calculated for each degree of β-turn bias, and the results were compared with analytical ultracentrifugation experiments at various temperatures. We find that the temperature dependence of the sedimentation coefficient (s20,wo) can be reproduced by increasing the β-turn content in the structural ensemble. This analysis allows us to estimate the presence of β-turns and weak associations under experimental conditions. Because disordered proteins frequently exhibit weak biases in secondary structure propensity, these experimentally-driven ensemble calculations may complement existing methods for modeling disordered proteins generally." @default.
• W2797783923 created "2018-04-24" @default.
• W2797783923 creator A5001318295 @default.
• W2797783923 creator A5006875674 @default.
• W2797783923 creator A5034262384 @default.
• W2797783923 creator A5038982579 @default.
• W2797783923 creator A5055411327 @default.
• W2797783923 creator A5078860033 @default.
• W2797783923 creator A5089260966 @default.
• W2797783923 date "2018-04-01" @default.
• W2797783923 modified "2023-10-13" @default.
• W2797783923 title "Modeling the Early Stages of Phase Separation in Disordered Elastin-like Proteins" @default.
• W2797783923 cites W1490138956 @default.
• W2797783923 cites W1515778899 @default.
• W2797783923 cites W1516874380 @default.
• W2797783923 cites W1586022877 @default.
• W2797783923 cites W1587690313 @default.
• W2797783923 cites W1823263094 @default.
• W2797783923 cites W1852070521 @default.
• W2797783923 cites W1896740278 @default.
• W2797783923 cites W1965127482 @default.
• W2797783923 cites W1968712781 @default.
• W2797783923 cites W1969749903 @default.
• W2797783923 cites W1970147513 @default.
• W2797783923 cites W1971594270 @default.
• W2797783923 cites W1971780729 @default.
• W2797783923 cites W1972598474 @default.
• W2797783923 cites W1978313975 @default.
• W2797783923 cites W1979483585 @default.
• W2797783923 cites W1981452730 @default.
• W2797783923 cites W1982383324 @default.
• W2797783923 cites W1982941857 @default.
• W2797783923 cites W1985681646 @default.
• W2797783923 cites W1988900301 @default.
• W2797783923 cites W1994057597 @default.
• W2797783923 cites W1994633856 @default.
• W2797783923 cites W2001900040 @default.
• W2797783923 cites W2002732486 @default.
• W2797783923 cites W2002900623 @default.
• W2797783923 cites W2003390427 @default.
• W2797783923 cites W2008846230 @default.
• W2797783923 cites W2010631859 @default.
• W2797783923 cites W2010753316 @default.
• W2797783923 cites W2013491063 @default.
• W2797783923 cites W2013931680 @default.
• W2797783923 cites W2014541739 @default.
• W2797783923 cites W2021804758 @default.
• W2797783923 cites W2022564067 @default.
• W2797783923 cites W2026729332 @default.
• W2797783923 cites W2026946969 @default.
• W2797783923 cites W2027473058 @default.
• W2797783923 cites W2029061185 @default.
• W2797783923 cites W2030728145 @default.
• W2797783923 cites W2031330956 @default.
• W2797783923 cites W2036824614 @default.
• W2797783923 cites W2038277098 @default.
• W2797783923 cites W2041091991 @default.
• W2797783923 cites W2041368279 @default.
• W2797783923 cites W2042745266 @default.
• W2797783923 cites W2043556712 @default.
• W2797783923 cites W2050625197 @default.
• W2797783923 cites W2051824584 @default.
• W2797783923 cites W2052606462 @default.
• W2797783923 cites W2056734463 @default.
• W2797783923 cites W2058789334 @default.
• W2797783923 cites W2059015279 @default.
• W2797783923 cites W2068220314 @default.
• W2797783923 cites W2068225333 @default.
• W2797783923 cites W2068994001 @default.
• W2797783923 cites W2071697435 @default.
• W2797783923 cites W2072259830 @default.
• W2797783923 cites W2076742999 @default.
• W2797783923 cites W2080896529 @default.
• W2797783923 cites W2082954900 @default.
• W2797783923 cites W2085362444 @default.
• W2797783923 cites W2085516160 @default.
• W2797783923 cites W2085978470 @default.
• W2797783923 cites W2088848851 @default.
• W2797783923 cites W2093214796 @default.
• W2797783923 cites W2110113231 @default.
• W2797783923 cites W2111113741 @default.
• W2797783923 cites W2114823344 @default.
• W2797783923 cites W2117238666 @default.
• W2797783923 cites W2120183443 @default.
• W2797783923 cites W2123743163 @default.
• W2797783923 cites W2132820390 @default.
• W2797783923 cites W2136240587 @default.
• W2797783923 cites W2136956472 @default.
• W2797783923 cites W2142808579 @default.
• W2797783923 cites W2148521301 @default.
• W2797783923 cites W2153153865 @default.
• W2797783923 cites W2153983456 @default.
• W2797783923 cites W2156771958 @default.
• W2797783923 cites W2160050027 @default.
• W2797783923 cites W2169821755 @default.
• W2797783923 cites W2171057750 @default.
• W2797783923 cites W2201344236 @default.
• W2797783923 cites W2300351299 @default.

• next