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• W2798082037 abstract "Extended-focus optical coherence tomography (xf-OCT) is a variant of optical coherence tomography (OCT) wherein the illumination and/or detection modes are engineered to provide a constant diffractionless lateral resolution over an extended depth of field (typically 3 to 10× the Rayleigh range). xf-OCT systems operating at 800 nm have been devised and used in the past to image brain structures at high-resolution in vivo, but are limited to ∼500 μm in penetration depth due to their short illumination wavelength. Here we present an xf-OCT system optimized to an image deeper within the cortex by using a longer illumination central wavelength of 1310 nm. The system offers a lateral resolution of 3 and 6.5 μm, over a depth of 900 μm and >1.5 mm using a 10× and 5× objective, respectively, in air. We characterize the system's resolution using microbeads embedded in PDMS and demonstrate its capabilities by imaging the cortical structure and microvasculature in anesthetized mice to a depth of ∼0.8 mm. Finally, we illustrate the difference in penetration depths obtainable with the new system and an xf-OCT system operating at 800 nm." @default.
• W2798082037 created "2018-04-24" @default.
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• W2798082037 date "2018-04-10" @default.
• W2798082037 modified "2023-10-10" @default.
• W2798082037 title "Imaging of cortical structures and microvasculature using extended-focus optical coherence tomography at 13 μm" @default.
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• W2798082037 doi "https://doi.org/10.1364/ol.43.001782" @default.
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• W2798082037 hasPublicationYear "2018" @default.
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