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- W2798261010 abstract "The exact pathways of collagen remodeling in tendon tissue are not well understood. Therefore, we have established an ex vivo 3D collagen gel-based system and we studied the remodeling capacity of two different TSPC lines from young, Y-TSPC and aged/degenerative, A-TSPC donors. Here, we specifically focused on investigating the involvement of integrin receptors in the remodeling process. Integrins are transmembrane receptors consisting of alpha (a) and beta (b) subunits, which form cell-to-matrix bonds, activate various pathways and thereby control cell proliferation, differentiation and survival.Y- and A-TSPC were derived from human Achilles tendons and are fully described in Kohler et al. 2013. RT-PCR was used to assess the expression of collagen-binding integrins in the TSPC cultivated in collagen gels. Next, a1 and a11 integrins were silenced by stable lentiviral delivery of target-specific shRNA in the Y-TSPC. Control (con-shRNA), integrin (a1-shRNA) and integrin a11 (a11-shRNA) virus-containing supe..." @default.
- W2798261010 created "2018-04-24" @default.
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- W2798261010 date "2018-02-21" @default.
- W2798261010 modified "2023-09-23" @default.
- W2798261010 title "TENDON COLLAGEN REMODELLING: NOVEL PATHWAY IMPLEMENTED BY TENDON STEM/PROGENITOR CELLS (TSPC)" @default.
- W2798261010 hasPublicationYear "2018" @default.
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