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- W2798819744 abstract "Abstract Cryptococcus neoformans is a unicellular fungal pathogen that causes meningoencephalitis, killing hundreds of thousands of patients each year. Its most distinctive characteristic is a polysaccharide capsule that envelops the whole cell. It is the major virulence attribute and the antigen for serologic diagnosis. We have developed a method for easy observation of the capsule and its growth dynamics using the cell-separation reagent Percoll and differential interference contrast (DIC) microscopy. Percoll suspension is far less disruptive of cell physiology than methods relying on antibody binding to the capsule, and measurements made with it are equivalent with India ink. Time-lapse microscopy observations using this method suggest that during budding, a dividing cell can regulate whether the capsule polysaccharide it produces is deposited on the capsule of the bud or on its own. This observation has important implications for our understanding of the C. neoformans capsule induction process during budding. List of abbreviations and acronyms CSF Cerebrospinal fluid DIC Differential interference microscopy NA Numerical aperture CCD Charge-coupled device MM Minimal medium CIM CO 2 -independent medium MOPS 3-Morpholinopropane-1-sulfonic acid SD Standard deviation" @default.
- W2798819744 created "2018-05-07" @default.
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- W2798819744 date "2018-05-03" @default.
- W2798819744 modified "2023-09-28" @default.
- W2798819744 title "Opsonin-free, real-time imaging of Cryptococcus neoformans capsule during budding" @default.
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- W2798819744 doi "https://doi.org/10.1101/313429" @default.
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