FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2799133209> ?p ?o ?g. }

• W2799133209 abstract "Abstract With an ongoing threat posed by circulating zoonotic strains, new strategies are required to prepare for the next emergent coronavirus (CoV). Previously, groups had targeted conserved coronavirus proteins as a strategy to generate live-attenuated vaccine strains against current and future CoVs. With this in mind, we explored whether manipulation of CoV NSP16, a conserved 2’O methyltransferase (MTase), could provide a broad attenuation platform against future emergent strains. Using the SARS-CoV mouse model, a NSP16 mutant vaccine was evaluated for protection from heterologous challenge, efficacy in the aging host, and potential for reversion to pathogenesis. Despite some success, concerns for virulence in the aged and potential for reversion makes targeting NSP16 alone an untenable approach. However, combining a 2’O MTase mutation with a previously described CoV fidelity mutant produced a vaccine strain capable of protection from heterologous virus challenge, efficacy in aged mice, and no evidence for reversion. Together, the results indicate that targeting the CoV 2’O MTase in parallel with other conserved attenuating mutations may provide a platform strategy for rapidly generating live-attenuated coronavirus vaccines. Significance Emergent coronaviruses remain a significant threat to global public health and rapid response vaccine platforms are needed to stem future outbreaks. However, failure of many previous CoV vaccine formulations has clearly highlighted the need to test efficacy under different conditions and especially in vulnerable populations like the aged and immune-compromised. This study illustrates that despite success in young models, the NSP16 mutant carries too much risk for pathogenesis and reversion in vulnerable models to be used as a stand-alone vaccine strategy. Importantly, the NSP16 mutation can be paired with other attenuating approaches to provide robust protection from heterologous challenge and in vulnerable populations. Coupled with increased safety and reduced pathogenesis, the study highlights the potential for NSP16 attenuation as a major component of future live-attenuated coronavirus vaccines." @default.
• W2799133209 created "2018-05-07" @default.
• W2799133209 creator A5017501496 @default.
• W2799133209 creator A5028054915 @default.
• W2799133209 creator A5032751745 @default.
• W2799133209 creator A5041059730 @default.
• W2799133209 creator A5052239466 @default.
• W2799133209 creator A5052355885 @default.
• W2799133209 creator A5053476989 @default.
• W2799133209 creator A5059897573 @default.
• W2799133209 creator A5061514063 @default.
• W2799133209 creator A5068803211 @default.
• W2799133209 date "2018-04-28" @default.
• W2799133209 modified "2023-09-23" @default.
• W2799133209 title "Combination attenuation offers strategy for live-attenuated coronavirus vaccines" @default.
• W2799133209 cites W1963580683 @default.
• W2799133209 cites W1976483167 @default.
• W2799133209 cites W1984494056 @default.
• W2799133209 cites W1995367098 @default.
• W2799133209 cites W2037466316 @default.
• W2799133209 cites W2046153984 @default.
• W2799133209 cites W2051052628 @default.
• W2799133209 cites W2054076340 @default.
• W2799133209 cites W2074007523 @default.
• W2799133209 cites W2088138646 @default.
• W2799133209 cites W2092969802 @default.
• W2799133209 cites W2093356049 @default.
• W2799133209 cites W2125470890 @default.
• W2799133209 cites W2127938920 @default.
• W2799133209 cites W2129655352 @default.
• W2799133209 cites W2137888434 @default.
• W2799133209 cites W2151847720 @default.
• W2799133209 cites W2166867592 @default.
• W2799133209 cites W2195009776 @default.
• W2799133209 cites W2255243349 @default.
• W2799133209 cites W2298153446 @default.
• W2799133209 cites W2341388825 @default.
• W2799133209 cites W2466982071 @default.
• W2799133209 cites W2559337285 @default.
• W2799133209 cites W2601559170 @default.
• W2799133209 cites W2725497285 @default.
• W2799133209 cites W2747597014 @default.
• W2799133209 cites W2770116590 @default.
• W2799133209 cites W3121415489 @default.
• W2799133209 doi "https://doi.org/10.1101/309591" @default.
• W2799133209 hasPublicationYear "2018" @default.
• W2799133209 type Work @default.
• W2799133209 sameAs 2799133209 @default.
• W2799133209 citedByCount "3" @default.
• W2799133209 countsByYear W27991332092018 @default.
• W2799133209 countsByYear W27991332092019 @default.
• W2799133209 countsByYear W27991332092020 @default.
• W2799133209 crossrefType "posted-content" @default.
• W2799133209 hasAuthorship W2799133209A5017501496 @default.
• W2799133209 hasAuthorship W2799133209A5028054915 @default.
• W2799133209 hasAuthorship W2799133209A5032751745 @default.
• W2799133209 hasAuthorship W2799133209A5041059730 @default.
• W2799133209 hasAuthorship W2799133209A5052239466 @default.
• W2799133209 hasAuthorship W2799133209A5052355885 @default.
• W2799133209 hasAuthorship W2799133209A5053476989 @default.
• W2799133209 hasAuthorship W2799133209A5059897573 @default.
• W2799133209 hasAuthorship W2799133209A5061514063 @default.
• W2799133209 hasAuthorship W2799133209A5068803211 @default.
• W2799133209 hasBestOaLocation W27991332091 @default.
• W2799133209 hasConcept C104317684 @default.
• W2799133209 hasConcept C127716648 @default.
• W2799133209 hasConcept C142724271 @default.
• W2799133209 hasConcept C143065580 @default.
• W2799133209 hasConcept C159047783 @default.
• W2799133209 hasConcept C16318435 @default.
• W2799133209 hasConcept C203014093 @default.
• W2799133209 hasConcept C2777648638 @default.
• W2799133209 hasConcept C2779134260 @default.
• W2799133209 hasConcept C3008058167 @default.
• W2799133209 hasConcept C524204448 @default.
• W2799133209 hasConcept C54355233 @default.
• W2799133209 hasConcept C54602769 @default.
• W2799133209 hasConcept C60987743 @default.
• W2799133209 hasConcept C70883133 @default.
• W2799133209 hasConcept C71924100 @default.
• W2799133209 hasConcept C86803240 @default.
• W2799133209 hasConcept C8891405 @default.
• W2799133209 hasConceptScore W2799133209C104317684 @default.
• W2799133209 hasConceptScore W2799133209C127716648 @default.
• W2799133209 hasConceptScore W2799133209C142724271 @default.
• W2799133209 hasConceptScore W2799133209C143065580 @default.
• W2799133209 hasConceptScore W2799133209C159047783 @default.
• W2799133209 hasConceptScore W2799133209C16318435 @default.
• W2799133209 hasConceptScore W2799133209C203014093 @default.
• W2799133209 hasConceptScore W2799133209C2777648638 @default.
• W2799133209 hasConceptScore W2799133209C2779134260 @default.
• W2799133209 hasConceptScore W2799133209C3008058167 @default.
• W2799133209 hasConceptScore W2799133209C524204448 @default.
• W2799133209 hasConceptScore W2799133209C54355233 @default.
• W2799133209 hasConceptScore W2799133209C54602769 @default.
• W2799133209 hasConceptScore W2799133209C60987743 @default.
• W2799133209 hasConceptScore W2799133209C70883133 @default.
• W2799133209 hasConceptScore W2799133209C71924100 @default.
• W2799133209 hasConceptScore W2799133209C86803240 @default.
• W2799133209 hasConceptScore W2799133209C8891405 @default.

• next