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• W2799284807 abstract "Efficient intracellular delivery of target macromolecules remains a major obstacle in cell engineering and other biomedical applications. We discovered a unique cell biophysical phenomenon of transient cell volume exchange by using microfluidics to rapidly and repeatedly compress cells. This behavior consists of brief, mechanically induced cell volume loss followed by rapid volume recovery. We harness this behavior for high-throughput, convective intracellular delivery of large polysaccharides (2000 kDa), particles (100 nm), and plasmids while maintaining high cell viability. Successful proof of concept experiments in transfection and intracellular labeling demonstrated potential to overcome the most prohibitive challenges in intracellular delivery for cell engineering." @default.
• W2799284807 created "2018-05-17" @default.
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• W2799284807 date "2018-09-01" @default.
• W2799284807 modified "2023-10-05" @default.
• W2799284807 title "Microfluidic generation of transient cell volume exchange for convectively driven intracellular delivery of large macromolecules" @default.
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• W2799284807 doi "https://doi.org/10.1016/j.mattod.2018.03.002" @default.
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