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• W2799368420 abstract "Significance The family of G-protein–coupled receptors (GPCRs) is the largest receptor family, and each member detects specific ligands, which in turn, activate selected members of one or more G-protein families. We developed a broadly applicable assay to study the specificity of GPCR–G-protein interaction in a subtype selective manner. We were able to quantitatively measure both association and dissociation rates of G proteins from agonist-activated GPCRs. We found that the stability of the receptor–G-protein complex is an inherent property of both interaction partners independent on the agonist used. Furthermore, its lifetime correlated closely with the ability of the receptor to activate the corresponding G-protein subtype." @default.
• W2799368420 created "2018-05-17" @default.
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• W2799368420 date "2018-04-23" @default.
• W2799368420 modified "2023-10-16" @default.
• W2799368420 title "Lifetime of muscarinic receptor–G-protein complexes determines coupling efficiency and G-protein subtype selectivity" @default.
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• W2799368420 doi "https://doi.org/10.1073/pnas.1715751115" @default.
• W2799368420 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5948956" @default.
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