Matches in SemOpenAlex for { <https://semopenalex.org/work/W2799544024> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2799544024 endingPage "889" @default.
- W2799544024 startingPage "873" @default.
- W2799544024 abstract "Background/Aims The research of genes implicated in kidney glomerular function, eliciting cell fate program, is always at the forefront in nephrological studies. Several neurological molecules have been recently the object of study not only for their involvement in the central nervous system differentiation but also for their importance in the functionality of other organs and for mature phenotype, as in kidney. NeuroD, in CNS, is related to two functional roles, the early survival and the differentiation. The aim of our study was to ascertain the presence of NeuroD transcription factor in glomeruli and to understand which targets and mechanisms NeuroD controls. Methods: We used immunofluorescence (IF) studies on both human and mice renal tissues and on cultured podocytes to describe NeuroD distribution; then we investigated NeuroD binding to the nephrin promoter region in cultured podocytes by chromatin-immuno-precipitation (ChIP) assay. The overexpression of NeuroD in podocytes was used to establish first its role in nephrin synthesis, evaluated by real-time quantitative (RTq) PCR and western-blot (WB) and successively to determine the recovery of cell morphology after adriamycin injury, measuring foot processes length. Results: We identified NeuroD transcription factor in glomeruli, in the same cells positive for WT1 and synaptopodin, namely podocytes; subsequently we observed a differentiation dependent NeuroD distribution in cultured podocytes, and a consistent link of NeuroD with the Nephrin promoter leading to the regulation of Nephrin translation and transcription. Our data also describes NeuroD expression in cytoplasm as phosphoprotein linked to nephrin and actinin4. Preliminary experiments seem to indicate NeuroD involved in dynamics of cell shape regulation after adriamycin injury. Conclusion: we propose that NeuroD possess in podocytes a dual ability acting in the nucleus as a transcription factor and in cytoplasm stabilizing cell shape." @default.
- W2799544024 created "2018-05-17" @default.
- W2799544024 creator A5004804603 @default.
- W2799544024 creator A5011863838 @default.
- W2799544024 creator A5014970553 @default.
- W2799544024 creator A5049341852 @default.
- W2799544024 creator A5064594935 @default.
- W2799544024 creator A5070949543 @default.
- W2799544024 creator A5076992449 @default.
- W2799544024 date "2018-01-01" @default.
- W2799544024 modified "2023-09-23" @default.
- W2799544024 title "NeuroD Expression in Podocytes and Interrelationships with Nephrin at Both Nuclear and Cytoplasmic Sites" @default.
- W2799544024 doi "https://doi.org/10.1159/000488818" @default.
- W2799544024 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29669318" @default.
- W2799544024 hasPublicationYear "2018" @default.
- W2799544024 type Work @default.
- W2799544024 sameAs 2799544024 @default.
- W2799544024 citedByCount "3" @default.
- W2799544024 countsByYear W27995440242019 @default.
- W2799544024 countsByYear W27995440242021 @default.
- W2799544024 countsByYear W27995440242022 @default.
- W2799544024 crossrefType "journal-article" @default.
- W2799544024 hasAuthorship W2799544024A5004804603 @default.
- W2799544024 hasAuthorship W2799544024A5011863838 @default.
- W2799544024 hasAuthorship W2799544024A5014970553 @default.
- W2799544024 hasAuthorship W2799544024A5049341852 @default.
- W2799544024 hasAuthorship W2799544024A5064594935 @default.
- W2799544024 hasAuthorship W2799544024A5070949543 @default.
- W2799544024 hasAuthorship W2799544024A5076992449 @default.
- W2799544024 hasBestOaLocation W27995440241 @default.
- W2799544024 hasConcept C104317684 @default.
- W2799544024 hasConcept C153911025 @default.
- W2799544024 hasConcept C2776415932 @default.
- W2799544024 hasConcept C2777146197 @default.
- W2799544024 hasConcept C2778994924 @default.
- W2799544024 hasConcept C2779561371 @default.
- W2799544024 hasConcept C2780091579 @default.
- W2799544024 hasConcept C2780819817 @default.
- W2799544024 hasConcept C2910727860 @default.
- W2799544024 hasConcept C54355233 @default.
- W2799544024 hasConcept C83640560 @default.
- W2799544024 hasConcept C86339819 @default.
- W2799544024 hasConcept C86803240 @default.
- W2799544024 hasConcept C95444343 @default.
- W2799544024 hasConceptScore W2799544024C104317684 @default.
- W2799544024 hasConceptScore W2799544024C153911025 @default.
- W2799544024 hasConceptScore W2799544024C2776415932 @default.
- W2799544024 hasConceptScore W2799544024C2777146197 @default.
- W2799544024 hasConceptScore W2799544024C2778994924 @default.
- W2799544024 hasConceptScore W2799544024C2779561371 @default.
- W2799544024 hasConceptScore W2799544024C2780091579 @default.
- W2799544024 hasConceptScore W2799544024C2780819817 @default.
- W2799544024 hasConceptScore W2799544024C2910727860 @default.
- W2799544024 hasConceptScore W2799544024C54355233 @default.
- W2799544024 hasConceptScore W2799544024C83640560 @default.
- W2799544024 hasConceptScore W2799544024C86339819 @default.
- W2799544024 hasConceptScore W2799544024C86803240 @default.
- W2799544024 hasConceptScore W2799544024C95444343 @default.
- W2799544024 hasIssue "3" @default.
- W2799544024 hasLocation W27995440241 @default.
- W2799544024 hasLocation W27995440242 @default.
- W2799544024 hasLocation W27995440243 @default.
- W2799544024 hasOpenAccess W2799544024 @default.
- W2799544024 hasPrimaryLocation W27995440241 @default.
- W2799544024 hasRelatedWork W1788736447 @default.
- W2799544024 hasRelatedWork W1971573514 @default.
- W2799544024 hasRelatedWork W2055301466 @default.
- W2799544024 hasRelatedWork W2060601209 @default.
- W2799544024 hasRelatedWork W2139698846 @default.
- W2799544024 hasRelatedWork W2158641610 @default.
- W2799544024 hasRelatedWork W2171065603 @default.
- W2799544024 hasRelatedWork W2333517425 @default.
- W2799544024 hasRelatedWork W2369977446 @default.
- W2799544024 hasRelatedWork W2799544024 @default.
- W2799544024 hasVolume "46" @default.
- W2799544024 isParatext "false" @default.
- W2799544024 isRetracted "false" @default.
- W2799544024 magId "2799544024" @default.
- W2799544024 workType "article" @default.