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- W2799588672 abstract "Culture models have been used for many years to study both normal breast biology and how defects arising in breast cancer cells lead to disease. Unfortunately, culturing breast cells on tissue culture plastic does not provide the right environment for the cells to function as they do in vivo, because plastic dishes are extremely noncompliant, and they contain nonbiological substrata. Conclusions about breast cell behaviors under these conditions are therefore not relevant to their activities in tissue. Three-dimensional (3D) culture models for breast cells have been used for the last 40 years, and they are very successful in supplying suitable conditions for many of the cellular functions that occur in tissues. Recently, new 3D constructs have been developed that contain a much greater selectivity in the types of extracellular matrix component, and that are able to confer many appropriate phenotypic properties to breast cells. Here we describe the old and the new models, and include detailed methods for analyzing breast cell function." @default.
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- W2799588672 date "2018-01-01" @default.
- W2799588672 modified "2023-10-02" @default.
- W2799588672 title "Three-dimensional breast culture models" @default.
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- W2799588672 doi "https://doi.org/10.1016/b978-0-12-812636-3.00004-3" @default.
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