Matches in SemOpenAlex for { <https://semopenalex.org/work/W2799686534> ?p ?o ?g. }
- W2799686534 endingPage "1046" @default.
- W2799686534 startingPage "1042" @default.
- W2799686534 abstract "Purpose: To identify mycotoxin genes among clinical ocular isolates of Fusarium species and to correlate these with clinical outcomes of Fusarium keratitis. Methods: Fifty-four clinical isolates of Fusarium were retrieved from the Bascom Palmer Eye Institute Ocular Microbiology Laboratory data bank. Multiplex polymerase chain reactions were run to confirm the identification of Fusarium species [internal transcribed spacer sequence, translation elongation factor 1-α (TEF) and β-tubulin] and to detect the presence of genes encoding production of fumonisin B mycotoxins (FUM1 and FUM8) and trichothecene mycotoxins (deoxynivalenol and nivalenol). The presence or absence of mycotoxins was compared with patient outcomes. Results: Forty-three (79%) of the 54 isolates were confirmed as Fusarium species, by an internal transcribed spacer sequence in 3 (5.6%) and by TEF in 43 (79.6%) of the 54 isolates. Fumonisin biosynthetic gene 1 (FUM1) was detected in 57.4% (n = 31/54) of the Fusarium isolates. No FUM8, deoxynivalenol genes, and nivalenol genes were detected among these in the clinical isolates group. Initial best-corrected visual acuity ranged from 20/25 to 20/80 in the FUM1 gene-negative group and from 20/20 to light perception in the FUM1 gene-positive group. There was no difference in the time to cure between both groups. The presence of FUM1 genes in 5 fungal isolates seemed to be associated with progression to penetrating keratoplasty in the 5 patients from whom the fungi were isolated. Fusarium solani was recovered from all patients requiring penetrating keratoplasty. Conclusions: Fumonisin B biosynthetic gene 1 may be common among clinical Fusarium isolates and contribute to worse initial visual acuity and high-risk progression to penetrating keratoplasty." @default.
- W2799686534 created "2018-05-17" @default.
- W2799686534 creator A5046556477 @default.
- W2799686534 creator A5065318636 @default.
- W2799686534 creator A5071889184 @default.
- W2799686534 creator A5082180164 @default.
- W2799686534 creator A5090181184 @default.
- W2799686534 date "2018-05-01" @default.
- W2799686534 modified "2023-10-18" @default.
- W2799686534 title "Multiplex Polymerase Chain Reaction Assay for Screening of Mycotoxin Genes From Ocular Isolates of Fusarium species" @default.
- W2799686534 cites W1562178609 @default.
- W2799686534 cites W1624362833 @default.
- W2799686534 cites W1963966594 @default.
- W2799686534 cites W1980671995 @default.
- W2799686534 cites W1982533264 @default.
- W2799686534 cites W1984490916 @default.
- W2799686534 cites W1991753799 @default.
- W2799686534 cites W1995164099 @default.
- W2799686534 cites W1998719612 @default.
- W2799686534 cites W2016470388 @default.
- W2799686534 cites W2075794294 @default.
- W2799686534 cites W2076325773 @default.
- W2799686534 cites W2089334150 @default.
- W2799686534 cites W2094661688 @default.
- W2799686534 cites W2098517022 @default.
- W2799686534 cites W2099295686 @default.
- W2799686534 cites W2103472692 @default.
- W2799686534 cites W2105737227 @default.
- W2799686534 cites W2138012030 @default.
- W2799686534 cites W2138176711 @default.
- W2799686534 cites W2138437709 @default.
- W2799686534 cites W2138755900 @default.
- W2799686534 cites W2164437570 @default.
- W2799686534 cites W2171955831 @default.
- W2799686534 cites W2177161308 @default.
- W2799686534 cites W2207809461 @default.
- W2799686534 cites W4240020555 @default.
- W2799686534 doi "https://doi.org/10.1097/ico.0000000000001607" @default.
- W2799686534 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29757853" @default.
- W2799686534 hasPublicationYear "2018" @default.
- W2799686534 type Work @default.
- W2799686534 sameAs 2799686534 @default.
- W2799686534 citedByCount "4" @default.
- W2799686534 countsByYear W27996865342020 @default.
- W2799686534 countsByYear W27996865342021 @default.
- W2799686534 countsByYear W27996865342022 @default.
- W2799686534 countsByYear W27996865342023 @default.
- W2799686534 crossrefType "journal-article" @default.
- W2799686534 hasAuthorship W2799686534A5046556477 @default.
- W2799686534 hasAuthorship W2799686534A5065318636 @default.
- W2799686534 hasAuthorship W2799686534A5071889184 @default.
- W2799686534 hasAuthorship W2799686534A5082180164 @default.
- W2799686534 hasAuthorship W2799686534A5090181184 @default.
- W2799686534 hasConcept C104317684 @default.
- W2799686534 hasConcept C155794727 @default.
- W2799686534 hasConcept C2776690007 @default.
- W2799686534 hasConcept C2778342517 @default.
- W2799686534 hasConcept C2778394675 @default.
- W2799686534 hasConcept C2778431908 @default.
- W2799686534 hasConcept C2778867309 @default.
- W2799686534 hasConcept C2781188995 @default.
- W2799686534 hasConcept C2781429003 @default.
- W2799686534 hasConcept C49105822 @default.
- W2799686534 hasConcept C54355233 @default.
- W2799686534 hasConcept C59822182 @default.
- W2799686534 hasConcept C67905577 @default.
- W2799686534 hasConcept C84699730 @default.
- W2799686534 hasConcept C86803240 @default.
- W2799686534 hasConcept C89423630 @default.
- W2799686534 hasConcept C90583042 @default.
- W2799686534 hasConceptScore W2799686534C104317684 @default.
- W2799686534 hasConceptScore W2799686534C155794727 @default.
- W2799686534 hasConceptScore W2799686534C2776690007 @default.
- W2799686534 hasConceptScore W2799686534C2778342517 @default.
- W2799686534 hasConceptScore W2799686534C2778394675 @default.
- W2799686534 hasConceptScore W2799686534C2778431908 @default.
- W2799686534 hasConceptScore W2799686534C2778867309 @default.
- W2799686534 hasConceptScore W2799686534C2781188995 @default.
- W2799686534 hasConceptScore W2799686534C2781429003 @default.
- W2799686534 hasConceptScore W2799686534C49105822 @default.
- W2799686534 hasConceptScore W2799686534C54355233 @default.
- W2799686534 hasConceptScore W2799686534C59822182 @default.
- W2799686534 hasConceptScore W2799686534C67905577 @default.
- W2799686534 hasConceptScore W2799686534C84699730 @default.
- W2799686534 hasConceptScore W2799686534C86803240 @default.
- W2799686534 hasConceptScore W2799686534C89423630 @default.
- W2799686534 hasConceptScore W2799686534C90583042 @default.
- W2799686534 hasIssue "8" @default.
- W2799686534 hasLocation W27996865341 @default.
- W2799686534 hasLocation W27996865342 @default.
- W2799686534 hasOpenAccess W2799686534 @default.
- W2799686534 hasPrimaryLocation W27996865341 @default.
- W2799686534 hasRelatedWork W1577173300 @default.
- W2799686534 hasRelatedWork W1971536147 @default.
- W2799686534 hasRelatedWork W1973197874 @default.
- W2799686534 hasRelatedWork W1996812132 @default.
- W2799686534 hasRelatedWork W2020086218 @default.
- W2799686534 hasRelatedWork W2042449942 @default.