FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2799693553> ?p ?o ?g. }

• W2799693553 endingPage "1220" @default.
• W2799693553 startingPage "1220" @default.
• W2799693553 abstract "Improving the efficacy of gene therapy vectors is still an important goal toward the development of safe and efficient gene therapy treatments. S/MAR (scaffold/matrix attached region)-based vectors are maintained extra-chromosomally in numerous cell types, which is similar to viral-based vectors. Additionally, when established as an episome, they show a very high mitotic stability. In the present study we tested the idea that addition of an S/MAR element to a CFTR (cystic fibrosis transmembrane conductance regulator) expression vector, may allow the establishment of a CFTR episome in bronchial epithelial cells. Starting from the observation that the S/MAR vector pEPI-EGFP (enhanced green fluorescence protein) is maintained as an episome in human bronchial epithelial cells, we assembled the CFTR vector pBQ-S/MAR. This vector, transfected in bronchial epithelial cells with mutated CFTR, supported long term wt CFTR expression and activity, which in turn positively impacted on the assembly of tight junctions in polarized epithelial cells. Additionally, the recovery of intact pBQ-S/MAR, but not the parental vector lacking the S/MAR element, from transfected cells after extensive proliferation, strongly suggested that pBQ-S/MAR was established as an episome. These results add a new element, the S/MAR, that can be considered to improve the persistence and safety of gene therapy vectors for cystic fibrosis pulmonary disease." @default.
• W2799693553 created "2018-05-17" @default.
• W2799693553 creator A5019256300 @default.
• W2799693553 creator A5024430695 @default.
• W2799693553 creator A5024587881 @default.
• W2799693553 creator A5028416545 @default.
• W2799693553 creator A5042136525 @default.
• W2799693553 creator A5050684931 @default.
• W2799693553 creator A5068820892 @default.
• W2799693553 creator A5072178306 @default.
• W2799693553 creator A5078032532 @default.
• W2799693553 creator A5082786197 @default.
• W2799693553 creator A5084664831 @default.
• W2799693553 date "2018-04-17" @default.
• W2799693553 modified "2023-09-27" @default.
• W2799693553 title "Assembly and Functional Analysis of an S/MAR Based Episome with the Cystic Fibrosis Transmembrane Conductance Regulator Gene" @default.
• W2799693553 cites W1489106364 @default.
• W2799693553 cites W1922064584 @default.
• W2799693553 cites W1936135223 @default.
• W2799693553 cites W1966051132 @default.
• W2799693553 cites W1967446284 @default.
• W2799693553 cites W1972106091 @default.
• W2799693553 cites W1974796951 @default.
• W2799693553 cites W1977343467 @default.
• W2799693553 cites W1980578842 @default.
• W2799693553 cites W1981481637 @default.
• W2799693553 cites W1985292442 @default.
• W2799693553 cites W1991964554 @default.
• W2799693553 cites W1997835226 @default.
• W2799693553 cites W2004155358 @default.
• W2799693553 cites W2011340684 @default.
• W2799693553 cites W2013032180 @default.
• W2799693553 cites W2015189075 @default.
• W2799693553 cites W2023447778 @default.
• W2799693553 cites W2032510845 @default.
• W2799693553 cites W2037668631 @default.
• W2799693553 cites W2040107868 @default.
• W2799693553 cites W2044380511 @default.
• W2799693553 cites W2049687913 @default.
• W2799693553 cites W2061752094 @default.
• W2799693553 cites W2067065069 @default.
• W2799693553 cites W2068981717 @default.
• W2799693553 cites W2078078890 @default.
• W2799693553 cites W2078738195 @default.
• W2799693553 cites W2091964753 @default.
• W2799693553 cites W2095461413 @default.
• W2799693553 cites W2110968074 @default.
• W2799693553 cites W2116707205 @default.
• W2799693553 cites W2123269598 @default.
• W2799693553 cites W2124886725 @default.
• W2799693553 cites W2131095976 @default.
• W2799693553 cites W2142381454 @default.
• W2799693553 cites W2144981650 @default.
• W2799693553 cites W2162527922 @default.
• W2799693553 cites W2170032136 @default.
• W2799693553 cites W2413982706 @default.
• W2799693553 cites W2419518172 @default.
• W2799693553 cites W2513515736 @default.
• W2799693553 cites W2528914659 @default.
• W2799693553 cites W2549404374 @default.
• W2799693553 cites W2556722702 @default.
• W2799693553 cites W2612703977 @default.
• W2799693553 cites W2626312014 @default.
• W2799693553 cites W4211056322 @default.
• W2799693553 cites W4229867118 @default.
• W2799693553 cites W59993807 @default.
• W2799693553 doi "https://doi.org/10.3390/ijms19041220" @default.
• W2799693553 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5979583" @default.
• W2799693553 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29673202" @default.
• W2799693553 hasPublicationYear "2018" @default.
• W2799693553 type Work @default.
• W2799693553 sameAs 2799693553 @default.
• W2799693553 citedByCount "8" @default.
• W2799693553 countsByYear W27996935532019 @default.
• W2799693553 countsByYear W27996935532020 @default.
• W2799693553 countsByYear W27996935532021 @default.
• W2799693553 countsByYear W27996935532022 @default.
• W2799693553 countsByYear W27996935532023 @default.
• W2799693553 crossrefType "journal-article" @default.
• W2799693553 hasAuthorship W2799693553A5019256300 @default.
• W2799693553 hasAuthorship W2799693553A5024430695 @default.
• W2799693553 hasAuthorship W2799693553A5024587881 @default.
• W2799693553 hasAuthorship W2799693553A5028416545 @default.
• W2799693553 hasAuthorship W2799693553A5042136525 @default.
• W2799693553 hasAuthorship W2799693553A5050684931 @default.
• W2799693553 hasAuthorship W2799693553A5068820892 @default.
• W2799693553 hasAuthorship W2799693553A5072178306 @default.
• W2799693553 hasAuthorship W2799693553A5078032532 @default.
• W2799693553 hasAuthorship W2799693553A5082786197 @default.
• W2799693553 hasAuthorship W2799693553A5084664831 @default.
• W2799693553 hasBestOaLocation W27996935531 @default.
• W2799693553 hasConcept C104317684 @default.
• W2799693553 hasConcept C111599444 @default.
• W2799693553 hasConcept C153911025 @default.
• W2799693553 hasConcept C170493617 @default.
• W2799693553 hasConcept C24530287 @default.
• W2799693553 hasConcept C2776938444 @default.
• W2799693553 hasConcept C2778428886 @default.

• next