FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2799697493> ?p ?o ?g. }

• W2799697493 endingPage "2327" @default.
• W2799697493 startingPage "2315" @default.
• W2799697493 abstract "Lactate is one of the key waste metabolites of mammalian cell culture. High lactate levels are caused by high aerobic glycolysis, also known as the Warburg effect, and are usually associated with adverse culture performance. Therefore, reducing lactate accumulation has been an ongoing challenge in the cell culture development to improve growth, productivity, and process robustness. The pyruvate dehydrogenase complex (PDC) plays a crucial role for the fate of pyruvate, as it converts pyruvate to acetyl coenzyme A (acetyl-CoA). The PDC activity can be indirectly increased by inhibiting the PDC inhibitor, pyruvate dehydrogenase kinase, using dichloroacetate (DCA), resulting in less pyruvate being available for lactate formation. Here, Chinese hamster ovary cells were cultivated either with 5 mM DCA or without DCA in various batch and fed-batch bioreactor processes. In all cultures, DCA increased peak viable cell density (VCD), culture length and final antibody titer. The strongest effect was observed in a fed batch with media and glucose feeding in which peak VCD was increased by more than 50%, culture length was extended by more than 3 days, and the final antibody titer increased by more than twofold. In cultures with DCA, lactate production and glucose consumption during exponential growth were on average reduced by approximately 40% and 35%, respectively. Metabolic flux analysis showed reduced glycolytic fluxes, whereas fluxes in the tricarboxylic acid (TCA) cycle were not affected, suggesting that cultures with DCA use glucose more efficiently. In a proteomics analysis, only few proteins were identified as being differentially expressed, indicating that DCA acts on a posttranslational level. Antibody quality in terms of aggregation, charge variant, and glycosylation pattern was unaffected. Subsequent bioreactor experiments with sodium lactate and sodium chloride feeding indicated that lower osmolality, rather than lower lactate concentration itself, improved culture performance in DCA cultures. In conclusion, the addition of DCA to the cell culture improved culture performance and increased antibody titers without any disadvantages for cell-specific productivity or antibody quality." @default.
• W2799697493 created "2018-05-17" @default.
• W2799697493 creator A5030141585 @default.
• W2799697493 creator A5061172047 @default.
• W2799697493 creator A5065005971 @default.
• W2799697493 creator A5077967664 @default.
• W2799697493 date "2018-05-22" @default.
• W2799697493 modified "2023-09-30" @default.
• W2799697493 title "Improving culture performance and antibody production in CHO cell culture processes by reducing the Warburg effect" @default.
• W2799697493 cites W1587804137 @default.
• W2799697493 cites W1759931938 @default.
• W2799697493 cites W1972023214 @default.
• W2799697493 cites W1973372532 @default.
• W2799697493 cites W1974220469 @default.
• W2799697493 cites W1977595804 @default.
• W2799697493 cites W1979947502 @default.
• W2799697493 cites W1986130675 @default.
• W2799697493 cites W1994251377 @default.
• W2799697493 cites W1996365670 @default.
• W2799697493 cites W1996466755 @default.
• W2799697493 cites W2000065954 @default.
• W2799697493 cites W2002172128 @default.
• W2799697493 cites W2011646732 @default.
• W2799697493 cites W2014387943 @default.
• W2799697493 cites W2014577850 @default.
• W2799697493 cites W2015915283 @default.
• W2799697493 cites W2020840252 @default.
• W2799697493 cites W2021068795 @default.
• W2799697493 cites W2021308621 @default.
• W2799697493 cites W2021351846 @default.
• W2799697493 cites W2024753771 @default.
• W2799697493 cites W2030417256 @default.
• W2799697493 cites W2030837323 @default.
• W2799697493 cites W2032513246 @default.
• W2799697493 cites W2037656934 @default.
• W2799697493 cites W2038597127 @default.
• W2799697493 cites W2039395021 @default.
• W2799697493 cites W2057058708 @default.
• W2799697493 cites W2058589373 @default.
• W2799697493 cites W2061462107 @default.
• W2799697493 cites W2063965892 @default.
• W2799697493 cites W2068967275 @default.
• W2799697493 cites W2072451938 @default.
• W2799697493 cites W2075802407 @default.
• W2799697493 cites W2077710574 @default.
• W2799697493 cites W2077994957 @default.
• W2799697493 cites W2083583693 @default.
• W2799697493 cites W2084219873 @default.
• W2799697493 cites W2093999909 @default.
• W2799697493 cites W2098201181 @default.
• W2799697493 cites W2102322990 @default.
• W2799697493 cites W2105399208 @default.
• W2799697493 cites W2118540273 @default.
• W2799697493 cites W2125765649 @default.
• W2799697493 cites W2142963315 @default.
• W2799697493 cites W2143258723 @default.
• W2799697493 cites W2149145325 @default.
• W2799697493 cites W2151517915 @default.
• W2799697493 cites W2155819450 @default.
• W2799697493 cites W2488483130 @default.
• W2799697493 doi "https://doi.org/10.1002/bit.26724" @default.
• W2799697493 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29704441" @default.
• W2799697493 hasPublicationYear "2018" @default.
• W2799697493 type Work @default.
• W2799697493 sameAs 2799697493 @default.
• W2799697493 citedByCount "42" @default.
• W2799697493 countsByYear W27996974932018 @default.
• W2799697493 countsByYear W27996974932019 @default.
• W2799697493 countsByYear W27996974932020 @default.
• W2799697493 countsByYear W27996974932021 @default.
• W2799697493 countsByYear W27996974932022 @default.
• W2799697493 countsByYear W27996974932023 @default.
• W2799697493 crossrefType "journal-article" @default.
• W2799697493 hasAuthorship W2799697493A5030141585 @default.
• W2799697493 hasAuthorship W2799697493A5061172047 @default.
• W2799697493 hasAuthorship W2799697493A5065005971 @default.
• W2799697493 hasAuthorship W2799697493A5077967664 @default.
• W2799697493 hasConcept C109156525 @default.
• W2799697493 hasConcept C165135838 @default.
• W2799697493 hasConcept C170493617 @default.
• W2799697493 hasConcept C175656101 @default.
• W2799697493 hasConcept C181199279 @default.
• W2799697493 hasConcept C20251656 @default.
• W2799697493 hasConcept C2777318727 @default.
• W2799697493 hasConcept C2780854706 @default.
• W2799697493 hasConcept C54355233 @default.
• W2799697493 hasConcept C55493867 @default.
• W2799697493 hasConcept C62231903 @default.
• W2799697493 hasConcept C81885089 @default.
• W2799697493 hasConcept C86803240 @default.
• W2799697493 hasConcept C88099638 @default.
• W2799697493 hasConcept C9506865 @default.
• W2799697493 hasConceptScore W2799697493C109156525 @default.
• W2799697493 hasConceptScore W2799697493C165135838 @default.
• W2799697493 hasConceptScore W2799697493C170493617 @default.
• W2799697493 hasConceptScore W2799697493C175656101 @default.
• W2799697493 hasConceptScore W2799697493C181199279 @default.
• W2799697493 hasConceptScore W2799697493C20251656 @default.

• next