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• W2799712560 abstract "// Nicky D’Haene 1 , Quitterie Fontanges 1 , Nancy De Nève 1 , Oriane Blanchard 1 , Barbara Melendez 1 , Monique Delos 2 , Marie-Françoise Dehou 3 , Calliope Maris 1, 4 , Nathalie Nagy 5 , Emmanuel Rousseau 6 , Josse Vandenhove 7 , André Gilles 8 , Carine De Prez 9 , Laurine Verset 1, 10 , Marie-Paule Van Craynest 11 , Pieter Demetter 1 , Jean-Luc Van Laethem 12 , Isabelle Salmon 1 and Marie Le Mercier 1 1 Department of Pathology, Erasme Hospital, Université Libre de Bruxelles, Brussels, Belgium 2 Department of Pathology, CHU UCL Namur, Yvoir, Belgium 3 CMP Pathology Laboratory, Brussels, Belgium 4 Department of Pathology, Braine l´Alleud Waterloo Hospital, Braine l´Alleud, Belgium 5 Department of Pathology, Charleroi University Hospital, Charleroi, Belgium 6 Department of Pathology, Mouscron Hospital, Mouscron, Belgium 7 Department of Pathology, Sint Maria Hospital, Halle, Belgium 8 Department of Pathology, EPICURA Hospital, Frameries, Belgium 9 Department of Pathology, Brugmann University Hospital, Brussels, Belgium 10 CurePath, Jumet, Belgium 11 New LabPatho, Braine l´Alleud, Belgium 12 Department of Oncology, Erasme Hospital, Université Libre de Bruxelles, Brussels, Belgium Correspondence to: Nicky D’Haene, email: nicky.d.haene@erasme.ulb.ac.be Keywords: colorectal cancer; next-generation sequencing Abbreviations: CRC: Colorectal Cancer; NGS: Next-generation sequencing; FFPE: formalin-fixed paraffin-embedded; TAT: turnaround time Received: December 12, 2017      Accepted: March 17, 2018      Published: April 17, 2018 ABSTRACT International guidelines made RAS (KRAS and NRAS) status a prerequisite for the use of anti-EGFR agents for metastatic colorectal cancer (CRC) patients. Daily, new data emerges on the theranostic and prognostic role of molecular biomarkers; this is a strong incentive for a validated, sensitive, and broadly available molecular screening test. Next-generation sequencing (NGS) has begun to supplant other technologies for genomic profiling. We report here our 2 years of clinical practice using NGS results to guide therapeutic decisions. The Ion Torrent AmpliSeq colon/lung cancer panel, which allows mutation detection in 22 cancer-related genes, was prospectively used in clinical practice (BELAC ISO 15189 accredited method). The DNA of 741 formalin-fixed paraffin-embedded CRC tissues, including primary tumors and metastasis, was obtained from 14 different Belgian institutions and subjected to targeted NGS. Of the tumors tested, 98% (727) were successfully sequenced and 89% (650) harbored at least one mutation. KRAS, BRAF and NRAS mutations were found in 335 (46%), 78 (11%) and 32 (4%) samples, respectively. These mutation frequencies were consistent with those reported in public databases. Moreover, mutations and amplifications in potentially actionable genes were identified in 464 samples (64%), including mutations in PIK3CA (14%), ERBB2 (0.4%), AKT1 (0.6%), and MAP2K1 (0.1%), as well as amplifications of ERBB2 (0.3%) and EGFR (0.3%). The median turnaround time between reception of the sample in the laboratory and report release was 8 calendar days. Overall, the AmpliSeq colon/lung cancer panel was successfully applied in daily practice and provided reliable clinically relevant information for CRC patients." @default.
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• W2799712560 date "2018-04-17" @default.
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• W2799712560 title "Clinical application of targeted next-generation sequencing for colorectal cancer patients: a multicentric Belgian experience" @default.
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• W2799712560 doi "https://doi.org/10.18632/oncotarget.25099" @default.
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