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- W2799760015 abstract "Cold physical plasmas have been shown to eliminate multiple types of cancers in vitro and in vivo [1] Although being multi-component systems, evidence suggest plasmas to be active primarily via release of a plethora of reactive oxygen and nitrogen species (ROS/RNS). These species not only damage cells via oxidation of proteins and lipids but also trigger signaling cascades via redox relays. This redox signaling is vital for a number of processes, e.g. the formation and loading of major histocompatibility class I complexes (MHC-I). Once loaded with peptides and translocated to the cell surface, MHC-I molecules are recognized by cytotoxic T lymphocytes. In cancer, high numbers of these lymphocytes often correlate with a good prognosis due to their ability to specifically recognize and kill tumor cells. Understanding whether and how MHCI loading and expression is regulated via exogenous oxidants such as produced by cold plasmas is key to delineate possible immune-modulating effects of plasmas in onco-therapy. Intracellular proteins get degraded to peptides by the proteasome before they get transported to the endoplasmatic reticulum by antigen peptide transporter TAP1 and TAP2. In endoplasmatic reticulum the peptides get associated with MHC-class I molecules via a number of proteins, such as Tapasin, ERp57 and PDI [2, 3]. Histone deacetylase inhibitors, interferones and cytokines are able to enhance the expression of TAP1, TAP2, LMP2, LMP7, and Tapasin in association with increased expression of MHC class I [4-6]. Here, we treated melanoma cells in vitro with cold plasma and investigated subsequent effects on the antigen processing machinery. We confirmed cytotoxic effects of the plasma treatment, and measured an upregulation of MHC-I on the cell surface using flow cytometry. Subsequently, we investigated quantity and activity of several proteins crucial in the antigen processing machinery, such as TAP1, TAP2, Tapasin, and ERp57, and we observed a modulation of protein targets. Trichostatin A, Interferon γ, and IL-10 served as positive controls as they promote antigen processing and presentation [5]. Our results suggest a role of plasma-derived, exogenous reactive species in the regulation of antigen presentation of tumor cells important for mounting antitumor immune responses.." @default.
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- W2799760015 date "2018-02-01" @default.
- W2799760015 modified "2023-09-27" @default.
- W2799760015 title "Regulation Of Antigen-Presenting Machinery In Melanoma After Plasma Treatment" @default.
- W2799760015 doi "https://doi.org/10.1016/j.cpme.2017.12.014" @default.
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