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- W2799797378 abstract "The study was aimed at investigating the most responsive part of Moringa oleifera Lam. that can serve as explant for in vitro culture propagation procedure. The phytohormones, 2, 4-Dichlorophenoxyacetic acid (2,4-D) at concentrations of 0.0, 2.5, 5.0, 7.5 and 10.0mg/l and kinetin at concentrations of 0.0, 0.1 and 1.0mg/l, either singly or in combination were used for supplementing Murashige and Skoog’s medium. The test parts of M. oleifera plant (leaf, cotyledon and stem) were each used to initiate in vitro cultures. After 3 weeks of culture initiation, the effectiveness of the morphogenetic response was determined using an assigned callus intensity scale. By a way of comparison, the results obtained indicated that callus can be generated from all the test plant parts, with the leaf tissue being the most responsive. The optimal amount of phytohormone combination for media supplementation was 5 mg/l 2,4-D and 0.1 mg/l kinetin. The stem explant seemed to be the least responsive for callus production." @default.
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- W2799797378 date "2017-09-01" @default.
- W2799797378 modified "2023-09-27" @default.
- W2799797378 title "A comparison of callus production from Moringa oleifera Lam. leaf, cotyledon and stem explants using 2, 4-Dichlorophenoxyacetic acid and kinetin for media supplementation" @default.
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