FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2799822509> ?p ?o ?g. }

• W2799822509 abstract "OBJECTIVE: To determine whether microglial morphology is significantly altered by the presence of repeat-associated non-AUG (RAN) translated proteins in ALS.BACKGROUND: Microglia are known to play a critical role in non-cell autonomous neuronal death in ALS. Recent data have suggested patients with C9orf72 expansion have even greater alterations in microglial morphology than other ALS patients. The mechanism for this is unknown although similar alterations in microglia have been identified in other repeat expansion diseases (e.g., Huntington’s disease). Here, we examined the hypothesis that RAN proteins, which form p62-positive cytoplasmic aggregates in patients with C9orf72 expansion, lead to alterations in microglial morphology in ALS, independent of TDP-43 and other pathologies.DESIGN/METHODS: Cytoplasmic RAN proteins were detected in autopsy-derived cerebellar cortical tissue of 65 ALS patients using anti-p62 Lck ligand immunofluorescence. Microglial density was quantified by CD68 immunohistochemistry. Iba1 immunofluorescence images were used to calculate an automated measure of cell area (Image J) and these images were also given a semi-quantitative score. Non-parametric statistical testing was used to examine the relationship between microglial measures and the presence of RAN proteins, as well as site of disease onset, duration, and age.RESULTS: p62-positive inclusions were identified in 12 patients. These patients significantly differed from p62-negative patients with respect to disease duration (2.45 versus 3.4 years, p = 0.01), but did not differ with respect to either Iba1- or CD68-based measures of microglia. Microglial measures in cerebellum were also not associated with age, duration of disease, or site of onset.CONCLUSIONS: Heterogeneity of microglia is present in ALS cerebellar cortex but this is not associated with the presence of RAN proteins in cerebellar granule cells. However, microglial morphology does not imply functional status so further tissue-based studies are underway to examine microglial function in the setting of p62-positive RAN proteins and ALS. Disclosure: Dr. Cykowski has nothing to disclose. Dr. Powell has nothing to disclose. Dr. Appel has nothing to disclose. Dr. Chang has nothing to disclose. Dr. Appel has received research support from Neuraltus, Inc., and GlaxoSmithKline." @default.
• W2799822509 created "2018-05-17" @default.
• W2799822509 creator A5015630437 @default.
• W2799822509 creator A5039046390 @default.
• W2799822509 creator A5059365083 @default.
• W2799822509 creator A5067158932 @default.
• W2799822509 creator A5085931831 @default.
• W2799822509 date "2016-04-05" @default.
• W2799822509 modified "2023-09-23" @default.
• W2799822509 title "Microglial Morphology Is Not Altered by the Presence of RAN Proteins in the Cerebellar Cortex of ALS Patients (P6.228)" @default.
• W2799822509 hasPublicationYear "2016" @default.
• W2799822509 type Work @default.
• W2799822509 sameAs 2799822509 @default.
• W2799822509 citedByCount "0" @default.
• W2799822509 crossrefType "journal-article" @default.
• W2799822509 hasAuthorship W2799822509A5015630437 @default.
• W2799822509 hasAuthorship W2799822509A5039046390 @default.
• W2799822509 hasAuthorship W2799822509A5059365083 @default.
• W2799822509 hasAuthorship W2799822509A5067158932 @default.
• W2799822509 hasAuthorship W2799822509A5085931831 @default.
• W2799822509 hasConcept C142724271 @default.
• W2799822509 hasConcept C159654299 @default.
• W2799822509 hasConcept C169760540 @default.
• W2799822509 hasConcept C186198217 @default.
• W2799822509 hasConcept C190062978 @default.
• W2799822509 hasConcept C203014093 @default.
• W2799822509 hasConcept C204232928 @default.
• W2799822509 hasConcept C2776460901 @default.
• W2799822509 hasConcept C2776914184 @default.
• W2799822509 hasConcept C2778236600 @default.
• W2799822509 hasConcept C2779134260 @default.
• W2799822509 hasConcept C2779652256 @default.
• W2799822509 hasConcept C2779830541 @default.
• W2799822509 hasConcept C2992333117 @default.
• W2799822509 hasConcept C502032728 @default.
• W2799822509 hasConcept C71924100 @default.
• W2799822509 hasConcept C86803240 @default.
• W2799822509 hasConcept C95444343 @default.
• W2799822509 hasConceptScore W2799822509C142724271 @default.
• W2799822509 hasConceptScore W2799822509C159654299 @default.
• W2799822509 hasConceptScore W2799822509C169760540 @default.
• W2799822509 hasConceptScore W2799822509C186198217 @default.
• W2799822509 hasConceptScore W2799822509C190062978 @default.
• W2799822509 hasConceptScore W2799822509C203014093 @default.
• W2799822509 hasConceptScore W2799822509C204232928 @default.
• W2799822509 hasConceptScore W2799822509C2776460901 @default.
• W2799822509 hasConceptScore W2799822509C2776914184 @default.
• W2799822509 hasConceptScore W2799822509C2778236600 @default.
• W2799822509 hasConceptScore W2799822509C2779134260 @default.
• W2799822509 hasConceptScore W2799822509C2779652256 @default.
• W2799822509 hasConceptScore W2799822509C2779830541 @default.
• W2799822509 hasConceptScore W2799822509C2992333117 @default.
• W2799822509 hasConceptScore W2799822509C502032728 @default.
• W2799822509 hasConceptScore W2799822509C71924100 @default.
• W2799822509 hasConceptScore W2799822509C86803240 @default.
• W2799822509 hasConceptScore W2799822509C95444343 @default.
• W2799822509 hasOpenAccess W2799822509 @default.
• W2799822509 hasRelatedWork W116889390 @default.
• W2799822509 hasRelatedWork W1458248728 @default.
• W2799822509 hasRelatedWork W1966166173 @default.
• W2799822509 hasRelatedWork W2086943561 @default.
• W2799822509 hasRelatedWork W2135280955 @default.
• W2799822509 hasRelatedWork W2542103564 @default.
• W2799822509 hasRelatedWork W2793632047 @default.
• W2799822509 hasRelatedWork W2895982335 @default.
• W2799822509 hasRelatedWork W2905818869 @default.
• W2799822509 hasRelatedWork W2932533304 @default.
• W2799822509 hasRelatedWork W2982367931 @default.
• W2799822509 hasRelatedWork W3005264352 @default.
• W2799822509 hasRelatedWork W3014092419 @default.
• W2799822509 hasRelatedWork W3043193619 @default.
• W2799822509 hasRelatedWork W3094177121 @default.
• W2799822509 hasRelatedWork W3135402083 @default.
• W2799822509 hasRelatedWork W3176378788 @default.
• W2799822509 hasRelatedWork W3180483827 @default.
• W2799822509 hasRelatedWork W3183396936 @default.
• W2799822509 hasRelatedWork W2574201760 @default.
• W2799822509 hasVolume "86" @default.
• W2799822509 isParatext "false" @default.
• W2799822509 isRetracted "false" @default.
• W2799822509 magId "2799822509" @default.
• W2799822509 workType "article" @default.