Matches in SemOpenAlex for { <https://semopenalex.org/work/W2799827358> ?p ?o ?g. }
Showing items 1 to 60 of
60
with 100 items per page.
- W2799827358 endingPage "S23" @default.
- W2799827358 startingPage "S23" @default.
- W2799827358 abstract "Mesenchymal stem cell-derived exosomes (MSC exosomes), nano-sized (30–200 nm in diameter) extracellular vesicles, are widely recognized as a potential cell-free therapeutic modality since they deliver a variety proteins, nucleic acids, and lipids from MSC. In addition, many studies have been currently on the way to utilize exosomes for diagnosis, drug delivery, and so on. However, large-scale production and isolation of exosomes from conditioned media with high purity is a major obstacle to overcome before development of exosome-based therapeutics. Conventional exosome isolation methods, such as ultracentrifugation, polymer-based precipitation, immunoaffinity purification, and ultrafiltration, have been limited by low purity, low yield, low throughput, or high cost. More importantly, scalability and GMP compliance are major issues that need to be addressed. In the present study, we successfully isolated MSC exosomes with high purity and homogeneity by our proprietary exosome isolation methods. The isolated exosomes were characterized as recommended by the International Society for Extracellular Vesicles (ISEV). To ensure the functionality of isolated exosomes, we also established an in vitro cell-based potency assay. Multiple batches of isolated exosomes can be easily tested and compared with this in vitro assay. Functionality of isolated exosomes is also confirmed through multiple isolations of single batch and multiple campaigns. Taken together, our proprietary method provides stable and scalable isolation of exosome from MSC conditioned media." @default.
- W2799827358 created "2018-05-17" @default.
- W2799827358 creator A5012228140 @default.
- W2799827358 creator A5018057046 @default.
- W2799827358 creator A5041228849 @default.
- W2799827358 date "2018-05-01" @default.
- W2799827358 modified "2023-10-18" @default.
- W2799827358 title "The stable production and isolation of exosome from human adipose tissue-derived mesenchymal stem cells" @default.
- W2799827358 doi "https://doi.org/10.1016/j.jcyt.2018.02.054" @default.
- W2799827358 hasPublicationYear "2018" @default.
- W2799827358 type Work @default.
- W2799827358 sameAs 2799827358 @default.
- W2799827358 citedByCount "0" @default.
- W2799827358 crossrefType "journal-article" @default.
- W2799827358 hasAuthorship W2799827358A5012228140 @default.
- W2799827358 hasAuthorship W2799827358A5018057046 @default.
- W2799827358 hasAuthorship W2799827358A5041228849 @default.
- W2799827358 hasConcept C104317684 @default.
- W2799827358 hasConcept C145059251 @default.
- W2799827358 hasConcept C185592680 @default.
- W2799827358 hasConcept C198826908 @default.
- W2799827358 hasConcept C20518536 @default.
- W2799827358 hasConcept C2778120352 @default.
- W2799827358 hasConcept C2781261824 @default.
- W2799827358 hasConcept C2908689518 @default.
- W2799827358 hasConcept C55493867 @default.
- W2799827358 hasConcept C86803240 @default.
- W2799827358 hasConcept C95444343 @default.
- W2799827358 hasConceptScore W2799827358C104317684 @default.
- W2799827358 hasConceptScore W2799827358C145059251 @default.
- W2799827358 hasConceptScore W2799827358C185592680 @default.
- W2799827358 hasConceptScore W2799827358C198826908 @default.
- W2799827358 hasConceptScore W2799827358C20518536 @default.
- W2799827358 hasConceptScore W2799827358C2778120352 @default.
- W2799827358 hasConceptScore W2799827358C2781261824 @default.
- W2799827358 hasConceptScore W2799827358C2908689518 @default.
- W2799827358 hasConceptScore W2799827358C55493867 @default.
- W2799827358 hasConceptScore W2799827358C86803240 @default.
- W2799827358 hasConceptScore W2799827358C95444343 @default.
- W2799827358 hasIssue "5" @default.
- W2799827358 hasLocation W27998273581 @default.
- W2799827358 hasOpenAccess W2799827358 @default.
- W2799827358 hasPrimaryLocation W27998273581 @default.
- W2799827358 hasRelatedWork W2057713753 @default.
- W2799827358 hasRelatedWork W2509815162 @default.
- W2799827358 hasRelatedWork W2785527513 @default.
- W2799827358 hasRelatedWork W2810395966 @default.
- W2799827358 hasRelatedWork W2906374810 @default.
- W2799827358 hasRelatedWork W2955875101 @default.
- W2799827358 hasRelatedWork W3139087999 @default.
- W2799827358 hasRelatedWork W3198772284 @default.
- W2799827358 hasRelatedWork W4238096637 @default.
- W2799827358 hasRelatedWork W4376112206 @default.
- W2799827358 hasVolume "20" @default.
- W2799827358 isParatext "false" @default.
- W2799827358 isRetracted "false" @default.
- W2799827358 magId "2799827358" @default.
- W2799827358 workType "article" @default.