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- W2799893482 abstract "It is well known that ion-pairing reagents cause ion suppression in LC-MS/MS methods. Here, we report that trichloroacetic acid increases the MS signal of tobramycin. To support studies of an in vitro pharmacokinetic/pharmacodynamic simulator for bacterial biofilms, an LC-MS/MS method for determination of tobramycin in M 9 media was developed. Aliquots of 25 <mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML id=M1><mml:mrow><mml:mi>μ</mml:mi></mml:mrow></mml:math>L M 9 media samples were mixed with the internal standard (IS) tobramycin-d 5 (5 µ g/mL, 25 µ L) and 200 µ L 2.5% trichloroacetic acid. The mixture (5 µ L) was directly injected onto a PFP column (2.0 × 50 mm, 3 µ m) eluted with water containing 20 mM ammonium formate and 0.14% trifluoroacetic acid and acetonitrile containing 0.1% trifluoroacetic acid in a gradient mode. ESI + and MRM with ion m / z 468 → 324 for tobramycin and m / z 473 → 327 for the IS were used for quantification. The calibration curve concentration range was 50–25000 ng/mL. Matrix effect from M 9 media was not significant when compared with injection solvents, but signal enhancement by trichloroacetic acid was significant (∼3 fold). The method is simple, fast, and reliable. Using the method, the in vitro PK/PD model was tested with one bolus dose of tobramycin." @default.
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- W2799893482 date "2018-01-01" @default.
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- W2799893482 title "Determination of Tobramycin in M<sub>9</sub> Medium by LC-MS/MS: Signal Enhancement by Trichloroacetic Acid" @default.
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- W2799893482 doi "https://doi.org/10.1155/2018/7965124" @default.
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