Matches in SemOpenAlex for { <https://semopenalex.org/work/W2800041319> ?p ?o ?g. }
- W2800041319 endingPage "2127" @default.
- W2800041319 startingPage "2113" @default.
- W2800041319 abstract "The N-terminally myristoylated matrix (MA) domain of the HIV-1 Gag polyprotein promotes virus assembly by targeting Gag to the inner leaflet of the plasma membrane. Recent studies indicate that, prior to membrane binding, MA associates with cytoplasmic tRNAs (including tRNALys3), and in vitro studies of tRNA-dependent MA interactions with model membranes have led to proposals that competitive tRNA interactions contribute to membrane discrimination. We have characterized interactions between native, mutant, and unmyristylated (myr-) MA proteins and recombinant tRNALys3 by NMR spectroscopy and isothermal titration calorimetry. NMR experiments confirm that tRNALys3 interacts with a patch of basic residues that are also important for binding to the plasma membrane marker, phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2]. Unexpectedly, the affinity of MA for tRNALys3 (Kd = 0.63 ± 0.03 μM) is approximately 1 order of magnitude greater than its affinity for PI(4,5)P2-enriched liposomes (Kd(apparent) = 10.2 ± 2.1 μM), and NMR studies indicate that tRNALys3 binding blocks MA association with liposomes, including those enriched with PI(4,5)P2, phosphatidylserine, and cholesterol. However, the affinity of MA for tRNALys3 is diminished by mutations or sample conditions that promote myristate exposure. Since Gag-Gag interactions are known to promote myristate exposure, our findings support virus assembly models in which membrane targeting and genome binding are mechanistically coupled." @default.
- W2800041319 created "2018-05-17" @default.
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- W2800041319 creator A5079194895 @default.
- W2800041319 creator A5090530236 @default.
- W2800041319 date "2018-07-01" @default.
- W2800041319 modified "2023-09-24" @default.
- W2800041319 title "HIV-1 Matrix Protein Interactions with tRNA: Implications for Membrane Targeting" @default.
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