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- W2800103712 abstract "Heterotrophic marine bacteria are challenged by versatile and dynamic nutrient conditions, which fluctuate seasonally, as a result of bloom and collapse of algae populations. Due to such conditions, the Roseobacter clade is one of the most abundant and metabolically active bacteria clades in marine environments.In this thesis, mainly GC-MS-based metabolomic analyses were applied to study how the metabolism of Phaeobacter inhibens DSM 17395 is adapted to the nutrient conditions in marine habitats. Collected data were statistically evaluated and interpreted in the context of collaborative performed complementary analyses.Phenotypic microarray analysis revealed a broad substrate range for carbon and nitrogen sources. Accordingly, cultivation in complex medium led to depletion of most of the provided substrates. Under these cultivation conditions, high abundance of catabolic enzymes and the corresponding intermediates were observed, which revealed simultaneous utilisation of different substrates, especially amino acids. To analyse their utilisation in a detailed study, a defined casamino acid medium was used for a time-dynamic resolution. Although different amino acids were utilised simultaneously, a specific uptake preference was observed. Further, even though sufficient amino acids were still available, cells entered stationary phase. In contrast to this, strains cured from the 262 kbp plasmid showed enhanced growth. Thus, either regulatory factors or the tropodithietic acid biosynthesis, which are encoded on this plasmid, are responsible for growth inhibition.Based on the original genome annotation, some amino acid (Phe, Lys, His, Met, Leu, Ile, Val, Thr and Trp) and carbohydrate degradation pathways (N-acetyl-glucosamine, mannitol, sucrose and xylose) remained unclear. Metabolomic and proteomic analyses, which were conducted in this study, allowed reconstruction of these pathways. Further, these analyses showed that P. inhibens DSM 17395 is well adapted to catabolise efficiently single substrates. But replenishment of the TCA cycle for anabolism lacked efficiency, because of usage of the complex ethylmalonyl-CoA pathway. This pathway is widely distributed in the Roseobacter clade and could completely be reconstructed by proteomic and LC-MS-based coenzyme A analysis.Overall, this thesis revealed many new metabolic features, which explain the adaptation of P. inhibens DSM 17395 to marine habitats and improve our understanding of marine bacteria." @default.
- W2800103712 created "2018-05-17" @default.
- W2800103712 creator A5036518352 @default.
- W2800103712 date "2015-03-05" @default.
- W2800103712 modified "2023-09-27" @default.
- W2800103712 title "Metabolic characterisation of the nutritional versatile marine bacterium Phaeobacter inhibens DSM 17395 via gas chromatography – mass spectrometry" @default.
- W2800103712 doi "https://doi.org/10.24355/dbbs.084-201504201100-0" @default.
- W2800103712 hasPublicationYear "2015" @default.
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