Matches in SemOpenAlex for { <https://semopenalex.org/work/W2800367049> ?p ?o ?g. }
- W2800367049 abstract "Abstract Transcription in bacteria is controlled by multiple molecular mechanisms that precisely regulate gene expression. It has been recently shown that initial RNA synthesis by the bacterial RNA polymerase (RNAP) is interrupted by pauses; however, the pausing determinants and the relationship of pausing with productive and abortive RNA synthesis remain poorly understood. Using single-molecule FRET and biochemical analysis, here we show that the pause encountered by RNAP after the synthesis of a 6-nt RNA (ITC6) renders the promoter escape strongly dependent on the NTP concentration. Mechanistically, the paused ITC6 acts as a checkpoint that directs RNAP to one of three competing pathways: productive transcription, abortive RNA release, or a new unscrunching/scrunching pathway. The cyclic unscrunching/scrunching of the promoter generates a long-lived, RNA-bound paused state; the abortive RNA release and DNA unscrunching are thus not as tightly linked as previously thought. Finally, our new model couples the pausing with the abortive and productive outcomes of initial transcription." @default.
- W2800367049 created "2018-05-17" @default.
- W2800367049 creator A5010786669 @default.
- W2800367049 creator A5011253940 @default.
- W2800367049 creator A5015470636 @default.
- W2800367049 creator A5016372894 @default.
- W2800367049 creator A5016910988 @default.
- W2800367049 creator A5020294036 @default.
- W2800367049 creator A5032923440 @default.
- W2800367049 creator A5042298609 @default.
- W2800367049 creator A5042649027 @default.
- W2800367049 creator A5053063044 @default.
- W2800367049 creator A5061313064 @default.
- W2800367049 date "2018-04-16" @default.
- W2800367049 modified "2023-09-30" @default.
- W2800367049 title "Pausing controls branching between productive and non-productive pathways during initial transcription in bacteria" @default.
- W2800367049 cites W1551727602 @default.
- W2800367049 cites W1590986325 @default.
- W2800367049 cites W1884241479 @default.
- W2800367049 cites W1887471033 @default.
- W2800367049 cites W1912050508 @default.
- W2800367049 cites W1964767569 @default.
- W2800367049 cites W1966114579 @default.
- W2800367049 cites W1970950317 @default.
- W2800367049 cites W1975232273 @default.
- W2800367049 cites W1979200451 @default.
- W2800367049 cites W1981195515 @default.
- W2800367049 cites W1982734185 @default.
- W2800367049 cites W1983911140 @default.
- W2800367049 cites W1986994983 @default.
- W2800367049 cites W1987227626 @default.
- W2800367049 cites W1993587122 @default.
- W2800367049 cites W1999434982 @default.
- W2800367049 cites W1999816995 @default.
- W2800367049 cites W2000057317 @default.
- W2800367049 cites W2001394772 @default.
- W2800367049 cites W2008442844 @default.
- W2800367049 cites W2010777538 @default.
- W2800367049 cites W2032974810 @default.
- W2800367049 cites W2036166114 @default.
- W2800367049 cites W2042070371 @default.
- W2800367049 cites W2045155739 @default.
- W2800367049 cites W2052094781 @default.
- W2800367049 cites W2053667739 @default.
- W2800367049 cites W2054714075 @default.
- W2800367049 cites W2055506322 @default.
- W2800367049 cites W2057854870 @default.
- W2800367049 cites W2064210252 @default.
- W2800367049 cites W2077107799 @default.
- W2800367049 cites W2079337189 @default.
- W2800367049 cites W2079991169 @default.
- W2800367049 cites W2084744901 @default.
- W2800367049 cites W2092792860 @default.
- W2800367049 cites W2101929794 @default.
- W2800367049 cites W2108804414 @default.
- W2800367049 cites W2109364306 @default.
- W2800367049 cites W2111441093 @default.
- W2800367049 cites W2117321459 @default.
- W2800367049 cites W2124663905 @default.
- W2800367049 cites W2125132050 @default.
- W2800367049 cites W2129315441 @default.
- W2800367049 cites W2140803417 @default.
- W2800367049 cites W2141985609 @default.
- W2800367049 cites W2143091625 @default.
- W2800367049 cites W2143975644 @default.
- W2800367049 cites W2151258156 @default.
- W2800367049 cites W2155581697 @default.
- W2800367049 cites W2157844474 @default.
- W2800367049 cites W2161729702 @default.
- W2800367049 cites W2168175751 @default.
- W2800367049 cites W2177374825 @default.
- W2800367049 cites W2199968103 @default.
- W2800367049 cites W2288550696 @default.
- W2800367049 cites W2315845140 @default.
- W2800367049 cites W2388875524 @default.
- W2800367049 cites W2400779101 @default.
- W2800367049 cites W2516060948 @default.
- W2800367049 cites W2519400805 @default.
- W2800367049 cites W2530360930 @default.
- W2800367049 cites W2535944182 @default.
- W2800367049 cites W2544758797 @default.
- W2800367049 cites W2597915083 @default.
- W2800367049 cites W2769280889 @default.
- W2800367049 doi "https://doi.org/10.1038/s41467-018-03902-9" @default.
- W2800367049 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5902446" @default.
- W2800367049 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29662062" @default.
- W2800367049 hasPublicationYear "2018" @default.
- W2800367049 type Work @default.
- W2800367049 sameAs 2800367049 @default.
- W2800367049 citedByCount "60" @default.
- W2800367049 countsByYear W28003670492018 @default.
- W2800367049 countsByYear W28003670492019 @default.
- W2800367049 countsByYear W28003670492020 @default.
- W2800367049 countsByYear W28003670492021 @default.
- W2800367049 countsByYear W28003670492022 @default.
- W2800367049 countsByYear W28003670492023 @default.
- W2800367049 crossrefType "journal-article" @default.
- W2800367049 hasAuthorship W2800367049A5010786669 @default.
- W2800367049 hasAuthorship W2800367049A5011253940 @default.
- W2800367049 hasAuthorship W2800367049A5015470636 @default.