FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2800417368> ?p ?o ?g. }

• W2800417368 endingPage "1138" @default.
• W2800417368 startingPage "1120" @default.
• W2800417368 abstract "As multiple species of bats are currently experiencing dramatic declines in populations due to white-nose syndrome (WNS) and other factors, conservation managers have an urgent need for data on the ecology and overall status of populations of once-common bat species. Standard approaches to obtain data on bat populations often involve capture and handling, requiring extensive expertise and unavoidably resulting in stress to the bats. New methods to rapidly obtain critical data are needed that minimize both the stress on bats and the spread of WNS. Guano provides a noninvasive source of DNA that includes information from the bat, but also dietary items, parasites, and pathogens. DNA metabarcoding is a high-throughput, DNA-based identification technique to assess the biodiversity of environmental or fecal samples. We investigated the use of multifaceted DNA metabarcoding (MDM), a technique combining next-generation DNA sequencing (NGS), DNA barcodes, and bioinformatic analysis, to simultaneously collect data on multiple parameters of interest (bat species composition, individual genotype, sex ratios, diet, parasites, and presence of WNS) from fecal samples using a single NGS run. We tested the accuracy of each MDM assay using samples in which these parameters were previously determined using conventional approaches. We found that assays for bat species identification, insect diet, parasite diversity, and genotype were both sensitive and accurate, the assay to detect WNS was highly sensitive but requires careful sample processing steps to ensure the reliability of results, while assays for nectivorous diet and sex showed lower sensitivity. MDM was able to quantify multiple data classes from fecal samples simultaneously, and results were consistent whether we included assays for a single data class or multiple data classes. Overall, MDM is a useful approach that employs noninvasive sampling and a customizable suite of assays to gain important and largely accurate information on bat ecology and population dynamics." @default.
• W2800417368 created "2018-05-17" @default.
• W2800417368 creator A5025898311 @default.
• W2800417368 creator A5031193698 @default.
• W2800417368 creator A5042248887 @default.
• W2800417368 creator A5052803150 @default.
• W2800417368 creator A5060410677 @default.
• W2800417368 creator A5075456988 @default.
• W2800417368 date "2018-05-31" @default.
• W2800417368 modified "2023-10-15" @default.
• W2800417368 title "Multifaceted <scp>DNA</scp> metabarcoding: Validation of a noninvasive, next‐generation approach to studying bat populations" @default.
• W2800417368 cites W1517341289 @default.
• W2800417368 cites W1539176226 @default.
• W2800417368 cites W1571552535 @default.
• W2800417368 cites W1807348612 @default.
• W2800417368 cites W1939400196 @default.
• W2800417368 cites W1959113564 @default.
• W2800417368 cites W1980416000 @default.
• W2800417368 cites W1987648496 @default.
• W2800417368 cites W1992648675 @default.
• W2800417368 cites W1994283123 @default.
• W2800417368 cites W2002682820 @default.
• W2800417368 cites W2013116967 @default.
• W2800417368 cites W2019016399 @default.
• W2800417368 cites W2021306682 @default.
• W2800417368 cites W2027738370 @default.
• W2800417368 cites W2028983737 @default.
• W2800417368 cites W2034134936 @default.
• W2800417368 cites W2037235346 @default.
• W2800417368 cites W2045295541 @default.
• W2800417368 cites W2051569068 @default.
• W2800417368 cites W2068040440 @default.
• W2800417368 cites W2075751076 @default.
• W2800417368 cites W2080040748 @default.
• W2800417368 cites W2093760700 @default.
• W2800417368 cites W2096525273 @default.
• W2800417368 cites W2098035523 @default.
• W2800417368 cites W2098423847 @default.
• W2800417368 cites W2098869000 @default.
• W2800417368 cites W2102408656 @default.
• W2800417368 cites W2102720559 @default.
• W2800417368 cites W2105022179 @default.
• W2800417368 cites W2107473082 @default.
• W2800417368 cites W2107474759 @default.
• W2800417368 cites W2110606082 @default.
• W2800417368 cites W2114259093 @default.
• W2800417368 cites W2121504024 @default.
• W2800417368 cites W2124532007 @default.
• W2800417368 cites W2130751261 @default.
• W2800417368 cites W2131985757 @default.
• W2800417368 cites W2133338472 @default.
• W2800417368 cites W2136254607 @default.
• W2800417368 cites W2138463603 @default.
• W2800417368 cites W2140227497 @default.
• W2800417368 cites W2142678478 @default.
• W2800417368 cites W2144361423 @default.
• W2800417368 cites W2149415648 @default.
• W2800417368 cites W2150228362 @default.
• W2800417368 cites W2151324874 @default.
• W2800417368 cites W2152189057 @default.
• W2800417368 cites W2152561898 @default.
• W2800417368 cites W2158918785 @default.
• W2800417368 cites W2164718269 @default.
• W2800417368 cites W2167572669 @default.
• W2800417368 cites W2168943441 @default.
• W2800417368 cites W2175294688 @default.
• W2800417368 cites W2175423925 @default.
• W2800417368 cites W2179942349 @default.
• W2800417368 cites W2231813633 @default.
• W2800417368 cites W2283535628 @default.
• W2800417368 cites W2334400755 @default.
• W2800417368 cites W2465681871 @default.
• W2800417368 cites W2509455153 @default.
• W2800417368 cites W2605840304 @default.
• W2800417368 cites W2627004953 @default.
• W2800417368 cites W2800417368 @default.
• W2800417368 cites W4234797890 @default.
• W2800417368 cites W3146967212 @default.
• W2800417368 doi "https://doi.org/10.1111/eva.12644" @default.
• W2800417368 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6050187" @default.
• W2800417368 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30026801" @default.
• W2800417368 hasPublicationYear "2018" @default.
• W2800417368 type Work @default.
• W2800417368 sameAs 2800417368 @default.
• W2800417368 citedByCount "27" @default.
• W2800417368 countsByYear W28004173682018 @default.
• W2800417368 countsByYear W28004173682019 @default.
• W2800417368 countsByYear W28004173682020 @default.
• W2800417368 countsByYear W28004173682021 @default.
• W2800417368 countsByYear W28004173682022 @default.
• W2800417368 countsByYear W28004173682023 @default.
• W2800417368 crossrefType "journal-article" @default.
• W2800417368 hasAuthorship W2800417368A5025898311 @default.
• W2800417368 hasAuthorship W2800417368A5031193698 @default.
• W2800417368 hasAuthorship W2800417368A5042248887 @default.
• W2800417368 hasAuthorship W2800417368A5052803150 @default.
• W2800417368 hasAuthorship W2800417368A5060410677 @default.
• W2800417368 hasAuthorship W2800417368A5075456988 @default.

• next