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- W2800465164 abstract "We have developed a novel in vitro research platform that combines a human hair-bearing skin explant (that retains the normal cellular organization interactions of the skin) with immunostaining of transverse human frozen skin sections of the hair follicle (HF) and interfollicular epidermis (IE). Fresh skin obtained from abdominoplasty from 3 healthy individuals was placed in explant standard (control) media or in media containing 12-o-tetradecanoylphorbol-13-acetate (TPA) (48 nm), a compound with attributed pro-proliferative effects on melanocytes (MCs). Biopsies were evaluated by immunostaining using fluorescent markers to identify PMEL(+) MC and KI-67(+) proliferative cells that were quantified by microscopy and cell counting. We show that skin explants incubated with standard media for 7 days reproduced the features of control skin [that was collected and frozen on day (D)0 and was not incubated with media]; these reproduced features were preserved architecture and similar expression of PMEL and of its proliferative and non-proliferative phenotypes, in both HFs and IE. On the D7-treated explants, TPA treatment was associated with remarkably increased number of PMEL(+) cells (P=2.0E-02), and of PMEL non-proliferative phenotype (P=3.0E-02), and with slightly increased number of PMEL proliferative cells (P=8.0E-02) in contrast to the to D7 explants incubated in control media. The above phenotypes had a similar expression trend in the upper HF infundibulum, but no treatment changes have been observed on the overall-depth analysis of HFs (P>0.05 for all 3 phenotypes). In summary, TPA treatment impacted the proliferation of mature MCs in the IE, but not the proliferation of the HF MC precursors. Our results suggest that ex vivo explants represent a valuable platform to reproduce the pharmacologic response of the in vivo human skin and will be a useful tool for testing candidate gene functions. Our goal is to study the effects of agonists on pathways activated in the HF and IE of vitiligo skin." @default.
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- W2800465164 date "2018-05-01" @default.
- W2800465164 modified "2023-10-16" @default.
- W2800465164 title "1250 Development of an ex vivo human skin explant model to examine candidate gene functions in the hair follicle and epidermis" @default.
- W2800465164 doi "https://doi.org/10.1016/j.jid.2018.03.1265" @default.
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