FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2800514662> ?p ?o ?g. }

• W2800514662 abstract "Mesenchymal stem cells (MSCs) produced for clinical purposes rely on culture media containing fetal bovine serum (FBS) which is xenogeneic and has the potential to significantly alter the MSC phenotype, rendering these cells immunogenic. As a result of bovine-derived exogenous proteins expressed on the cell surface, MSCs may be recognized by the host immune system as non-self and be rejected. Platelet lysate (PL) may obviate some of these concerns and shows promising results in human medicine as a possible alternative to FBS. Our goal was to evaluate the use of equine platelet lysate (ePL) pooled from donor horses in place of FBS to culture equine MSCs. We hypothesized that ePL, produced following apheresis, will function as the sole media supplement to accelerate the expansion of equine bone marrow-derived MSCs without altering their phenotype and their immunomodulatory capacity. Platelet concentrate was obtained via plateletpheresis and ePL were produced via freeze-thaw and centrifugation cycles. Population doublings (PD) and doubling time (DT) of bone marrow-derived MSCs (n = 3) cultured with FBS or ePL media were calculated. Cell viability, immunophenotypic analysis, and trilineage differentiation capacity of MSCs were assessed accordingly. To assess the ability of MSCs to modulate inflammatory responses, E. coli lipopolysaccharide (LPS)-stimulated monocytes were cocultured with MSCs cultured in the two different media formulations, and cell culture supernatants were assayed for the production of tumor necrosis factor (TNF)-α. Our results showed that MSCs cultured in ePL media exhibited similar proliferation rates (PD and DT) compared with those cultured in FBS at individual time points. MSCs cultured in ePL showed a statistically significant increased viability following a single washing step, expressed similar levels of MSC markers compared to FBS, and were able to differentiate towards the three lineages. Finally, MSCs cultured in ePL efficiently suppressed the release of TNF-α when exposed to LPS-stimulated monocytes similar to those cultured in FBS. ePL has the potential to be used for the expansion of MSCs before clinical application, avoiding the concerns associated with the use of FBS." @default.
• W2800514662 created "2018-05-17" @default.
• W2800514662 creator A5016255460 @default.
• W2800514662 creator A5018054675 @default.
• W2800514662 creator A5022558279 @default.
• W2800514662 creator A5024412611 @default.
• W2800514662 creator A5031483945 @default.
• W2800514662 creator A5064378694 @default.
• W2800514662 creator A5068773395 @default.
• W2800514662 creator A5070078657 @default.
• W2800514662 date "2018-03-22" @default.
• W2800514662 modified "2023-09-23" @default.
• W2800514662 title "Platelet lysate as a novel serum-free media supplement for the culture of equine bone marrow-derived mesenchymal stem cells" @default.
• W2800514662 cites W11305555 @default.
• W2800514662 cites W1496054330 @default.
• W2800514662 cites W1738380680 @default.
• W2800514662 cites W1825123972 @default.
• W2800514662 cites W1919767522 @default.
• W2800514662 cites W1969954863 @default.
• W2800514662 cites W1976173563 @default.
• W2800514662 cites W1981315552 @default.
• W2800514662 cites W1982422595 @default.
• W2800514662 cites W1982598271 @default.
• W2800514662 cites W1982666000 @default.
• W2800514662 cites W1982844650 @default.
• W2800514662 cites W1983713659 @default.
• W2800514662 cites W1984180661 @default.
• W2800514662 cites W1984909543 @default.
• W2800514662 cites W1993769261 @default.
• W2800514662 cites W1998373867 @default.
• W2800514662 cites W1999937740 @default.
• W2800514662 cites W2001895901 @default.
• W2800514662 cites W2010299188 @default.
• W2800514662 cites W2025371271 @default.
• W2800514662 cites W2026344537 @default.
• W2800514662 cites W2036791701 @default.
• W2800514662 cites W2037328562 @default.
• W2800514662 cites W2039713545 @default.
• W2800514662 cites W2041247926 @default.
• W2800514662 cites W2041971960 @default.
• W2800514662 cites W2045020549 @default.
• W2800514662 cites W2053976497 @default.
• W2800514662 cites W2058052934 @default.
• W2800514662 cites W2059380101 @default.
• W2800514662 cites W2061981400 @default.
• W2800514662 cites W2063596346 @default.
• W2800514662 cites W2066545720 @default.
• W2800514662 cites W2077027084 @default.
• W2800514662 cites W2081940406 @default.
• W2800514662 cites W2085048992 @default.
• W2800514662 cites W2087583213 @default.
• W2800514662 cites W2091128115 @default.
• W2800514662 cites W2091754993 @default.
• W2800514662 cites W2095643785 @default.
• W2800514662 cites W2109200717 @default.
• W2800514662 cites W2109587185 @default.
• W2800514662 cites W2112271653 @default.
• W2800514662 cites W2122366747 @default.
• W2800514662 cites W2127795633 @default.
• W2800514662 cites W2134682009 @default.
• W2800514662 cites W2136221233 @default.
• W2800514662 cites W2137242127 @default.
• W2800514662 cites W2148758648 @default.
• W2800514662 cites W2162749230 @default.
• W2800514662 cites W2166741387 @default.
• W2800514662 cites W2168800437 @default.
• W2800514662 cites W2170589477 @default.
• W2800514662 cites W2170868382 @default.
• W2800514662 cites W2171384041 @default.
• W2800514662 cites W2183709910 @default.
• W2800514662 cites W2213727944 @default.
• W2800514662 cites W2409721143 @default.
• W2800514662 cites W2493958312 @default.
• W2800514662 cites W2518015412 @default.
• W2800514662 cites W2524050293 @default.
• W2800514662 cites W2609906631 @default.
• W2800514662 cites W2770110590 @default.
• W2800514662 cites W4233591616 @default.
• W2800514662 doi "https://doi.org/10.1186/s13287-018-0823-3" @default.
• W2800514662 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5863827" @default.
• W2800514662 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29566772" @default.
• W2800514662 hasPublicationYear "2018" @default.
• W2800514662 type Work @default.
• W2800514662 sameAs 2800514662 @default.
• W2800514662 citedByCount "29" @default.
• W2800514662 countsByYear W28005146622019 @default.
• W2800514662 countsByYear W28005146622020 @default.
• W2800514662 countsByYear W28005146622021 @default.
• W2800514662 countsByYear W28005146622022 @default.
• W2800514662 countsByYear W28005146622023 @default.
• W2800514662 crossrefType "journal-article" @default.
• W2800514662 hasAuthorship W2800514662A5016255460 @default.
• W2800514662 hasAuthorship W2800514662A5018054675 @default.
• W2800514662 hasAuthorship W2800514662A5022558279 @default.
• W2800514662 hasAuthorship W2800514662A5024412611 @default.
• W2800514662 hasAuthorship W2800514662A5031483945 @default.
• W2800514662 hasAuthorship W2800514662A5064378694 @default.
• W2800514662 hasAuthorship W2800514662A5068773395 @default.
• W2800514662 hasAuthorship W2800514662A5070078657 @default.
• W2800514662 hasBestOaLocation W28005146621 @default.

• next