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- W2800563030 abstract "Recently Hage et al. reported six severe pneumonia cases infected with human adenovirus (HAdV) species B subtype 21a, 1 Hage E Huzly D Ganzenmueller T Beck R Schulz TF Heim A A human adenovirus species B subtype 21a associated with severe pneumonia. J Infect. 2014; 69: 490-499 Abstract Full Text Full Text PDF PubMed Scopus (26) Google Scholar a novel adenovirus type defined by its genome variation. Here, we detected HAdV in three severe pneumonia cases but failed to type with commonly used PCR method. We then subjected these samples to metagenomic sequencing and obtained the complete genome of the three HAdVs, allowing type identification of two B7 and one B3 in the tree cases. Further analysis identified variation occurred in the genome region targeted by PCR. Metagenomic sequencing also provided overall pathogens detected by traditional culture assays. This report highlights the robust power of metagenomic sequencing on the typing and genome sequencing of clinical pathogens." @default.
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- W2800563030 date "2018-08-01" @default.
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- W2800563030 title "Metagenomics facilitated complete genome sequencing of adenovirus untyped by real-time PCR" @default.
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- W2800563030 doi "https://doi.org/10.1016/j.jinf.2018.04.008" @default.
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