FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2800798857> ?p ?o ?g. }

• W2800798857 endingPage "047014" @default.
• W2800798857 startingPage "047014" @default.
• W2800798857 abstract "Background: Aromatase (CYP19) is a key enzyme in estrogens biosynthesis. In the mammary gland, CYP19 gene is expressed at low levels under the regulation of its I.4 promoter. In hormone-dependent breast cancer, fibroblast cells surrounding the tumor express increased levels of CYP19 mRNA due to a decrease of I.4 promoter activity and an increase of PII, I.3, and I.7 promoter activity. Little is known about the effects of environmental chemicals on the promoter-specific CYP19 expression. Objective: We aimed to determine the effects of two neonicotinoids (thiacloprid and imidacloprid) on promoter-specific CYP19 expression in Hs578t breast cancer cells and understand the signaling pathways involved. Methods: Hs578t cells were exposed to various signaling pathway stimulants or neonicotinoids for 24 h. Promoter-specific expression of CYP19 was determined by real-time quantitative polymerase chain reaction and catalytic activity of aromatase by tritiated water release assay. Results: To our knowledge, we are the first to demonstrate that the normal I.4 promoter and the breast cancer-relevant PII, I.3, and I.7 promoters of CYP19 are active in these cells. We found that the expression of CYP19 via promoters PII, I.3, and I.7 in Hs578t cells was, in part, dependent on the activation of two VEGF signaling pathways: mitogen-activated protein kinase (MAPK) 1/3 and phospholipase C (PLC). Exposure of Hs578t cells to environmental concentrations of imidacloprid and thiacloprid resulted in a switch in CYP19 promoter usage, involving inhibition of I.4 promoter activity and an increase of PII, I.3, and I.7 promoter-mediated CYP19 expression and aromatase catalytic activity. Greater effects were seen at lower concentrations. Our results suggest that thiacloprid and imidacloprid exert their effects at least partially by inducing the MAPK 1/3 and/or PLC pathways. Conclusions: We demonstrated in vitro that neonicotinoids may stimulate a change in CYP19 promoter usage similar to that observed in patients with hormone-dependent breast cancer. https://doi.org/10.1289/EHP2698" @default.
• W2800798857 created "2018-05-17" @default.
• W2800798857 creator A5013117243 @default.
• W2800798857 creator A5016915601 @default.
• W2800798857 creator A5079830300 @default.
• W2800798857 date "2018-04-05" @default.
• W2800798857 modified "2023-10-16" @default.
• W2800798857 title "Effects of Neonicotinoid Pesticides on Promoter-Specific Aromatase (CYP19) Expression in Hs578t Breast Cancer Cells and the Role of the VEGF Pathway" @default.
• W2800798857 cites W1492821183 @default.
• W2800798857 cites W1513652543 @default.
• W2800798857 cites W1555009525 @default.
• W2800798857 cites W1623983039 @default.
• W2800798857 cites W1888597759 @default.
• W2800798857 cites W1896458840 @default.
• W2800798857 cites W1964320715 @default.
• W2800798857 cites W1968377890 @default.
• W2800798857 cites W1969541824 @default.
• W2800798857 cites W1970323151 @default.
• W2800798857 cites W1970854096 @default.
• W2800798857 cites W1973335161 @default.
• W2800798857 cites W1984377226 @default.
• W2800798857 cites W1984858425 @default.
• W2800798857 cites W1990423138 @default.
• W2800798857 cites W1994664933 @default.
• W2800798857 cites W1996283549 @default.
• W2800798857 cites W2000769323 @default.
• W2800798857 cites W2000935008 @default.
• W2800798857 cites W2001044954 @default.
• W2800798857 cites W2010094441 @default.
• W2800798857 cites W2015229715 @default.
• W2800798857 cites W2017325045 @default.
• W2800798857 cites W2026963422 @default.
• W2800798857 cites W2031611218 @default.
• W2800798857 cites W2036801297 @default.
• W2800798857 cites W2045646084 @default.
• W2800798857 cites W2050725234 @default.
• W2800798857 cites W2055131068 @default.
• W2800798857 cites W2057939468 @default.
• W2800798857 cites W2061126156 @default.
• W2800798857 cites W2062809449 @default.
• W2800798857 cites W2066137744 @default.
• W2800798857 cites W2071062791 @default.
• W2800798857 cites W2072989635 @default.
• W2800798857 cites W2075335223 @default.
• W2800798857 cites W2075605442 @default.
• W2800798857 cites W2079668582 @default.
• W2800798857 cites W2080086312 @default.
• W2800798857 cites W2081709671 @default.
• W2800798857 cites W2090121445 @default.
• W2800798857 cites W2092171701 @default.
• W2800798857 cites W2092547974 @default.
• W2800798857 cites W2092708072 @default.
• W2800798857 cites W2100837885 @default.
• W2800798857 cites W2104345443 @default.
• W2800798857 cites W2105005092 @default.
• W2800798857 cites W2105053096 @default.
• W2800798857 cites W2106477645 @default.
• W2800798857 cites W2106668877 @default.
• W2800798857 cites W2109036715 @default.
• W2800798857 cites W2115576840 @default.
• W2800798857 cites W2126168000 @default.
• W2800798857 cites W2128098778 @default.
• W2800798857 cites W2133793175 @default.
• W2800798857 cites W2145526497 @default.
• W2800798857 cites W2145980235 @default.
• W2800798857 cites W2147124650 @default.
• W2800798857 cites W2147203478 @default.
• W2800798857 cites W2152142449 @default.
• W2800798857 cites W2158516918 @default.
• W2800798857 cites W2160480407 @default.
• W2800798857 cites W2165139205 @default.
• W2800798857 cites W2171087521 @default.
• W2800798857 cites W2258569606 @default.
• W2800798857 cites W2333776172 @default.
• W2800798857 cites W2738408208 @default.
• W2800798857 doi "https://doi.org/10.1289/ehp2698" @default.
• W2800798857 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6071809" @default.
• W2800798857 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29701941" @default.
• W2800798857 hasPublicationYear "2018" @default.
• W2800798857 type Work @default.
• W2800798857 sameAs 2800798857 @default.
• W2800798857 citedByCount "64" @default.
• W2800798857 countsByYear W28007988572018 @default.
• W2800798857 countsByYear W28007988572019 @default.
• W2800798857 countsByYear W28007988572020 @default.
• W2800798857 countsByYear W28007988572021 @default.
• W2800798857 countsByYear W28007988572022 @default.
• W2800798857 countsByYear W28007988572023 @default.
• W2800798857 crossrefType "journal-article" @default.
• W2800798857 hasAuthorship W2800798857A5013117243 @default.
• W2800798857 hasAuthorship W2800798857A5016915601 @default.
• W2800798857 hasAuthorship W2800798857A5079830300 @default.
• W2800798857 hasBestOaLocation W28007988571 @default.
• W2800798857 hasConcept C101762097 @default.
• W2800798857 hasConcept C104317684 @default.
• W2800798857 hasConcept C121608353 @default.
• W2800798857 hasConcept C150194340 @default.
• W2800798857 hasConcept C161176658 @default.

• next