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- W2800920834 abstract "Summary Lignified stone cells substantially reduce fruit quality. Therefore, it is desirable to inhibit stone cell development using genetic technologies. However, the molecular mechanisms regulating lignification are poorly understood in fruit stone cells. In this study, we have shown that micro RNA (miR) miR397a regulates fruit cell lignification by inhibiting laccase ( LAC ) genes that encode key lignin biosynthesis enzymes. Transient overexpression of PbrmiR397a , which is the miR397a of Chinese pear ( Pyrus bretschneideri ), and simultaneous silencing of three LAC genes reduced the lignin content and stone cell number in pear fruit. A single nucleotide polymorphism ( SNP ) identified in the promoter of the PbrmiR397a gene was found to associate with low levels of fruit lignin, after analysis of the genome sequences of sixty pear varieties. This SNP created a TCA element that responded to salicylic acid to induce gene expression as confirmed using a cell‐based assay system. Furthermore, stable overexpression of PbrmiR397a in transgenic tobacco plants reduced the expression of target LAC genes and decreased the content of lignin but did not change the ratio of syringyl‐ and guaiacyl‐lignin monomers. Consistent with reduction in lignin content, the transgenic plants showed fewer numbers of vessel elements and thinner secondary walls in the remaining elements compared to wild‐type control plants. This study has advanced our understanding of the regulation of lignin biosynthesis and provided useful molecular genetic information for improving pear fruit quality." @default.
- W2800920834 created "2018-05-17" @default.
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- W2800920834 date "2018-06-21" @default.
- W2800920834 modified "2023-10-01" @default.
- W2800920834 title "<i>PbrmiR397a</i> regulates lignification during stone cell development in pear fruit" @default.
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- W2800920834 doi "https://doi.org/10.1111/pbi.12950" @default.
- W2800920834 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6330545" @default.
- W2800920834 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29754465" @default.
- W2800920834 hasPublicationYear "2018" @default.
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