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- W2800959931 abstract "Barley malt contains hordein proteins, which gluten-sensitive consumers cannot tolerate. Beer produced from barley malt also contains hordeins. Aspergillus niger prolyl endoprotease (AN-PEP) is an enzyme that has been used effectively to reduce beer hordeins during fermentation. The objective of this study was to apply AN-PEP during the steeping and germination of barley and evaluate the impact on malt quality characteristics and the hordein content of model beers. Pilot-scale malting trials were performed, and the barley was germinated for either 3 or 5 days with and without AN-PEP. Model beers were produced from malts, and the levels of beer hordeins were tested using an R5 antibody-based competitive enzyme-linked immunosorbent assay. The malt friability, extract, viscosity, and several other quality parameters were measured following industry-standard MEBAK methods. Treatment of malt with AN-PEP for 5 days resulted in a 46% reduction in beer hordeins compared with beer produced from the 5 day control malt, and the quality of the AN-PEP treated malt was comparable to untreated malt. Applying enzymes to germinating grain is a novel way to influence the levels of hordeins in barley malt beers." @default.
- W2800959931 created "2018-05-17" @default.
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- W2800959931 date "2017-06-01" @default.
- W2800959931 modified "2023-09-30" @default.
- W2800959931 title "Reduction of Hordein Content in Beer by Applying Prolyl Endoprotease to the Malting Process" @default.
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- W2800959931 doi "https://doi.org/10.1094/asbcj-2017-3072-01" @default.
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